In vitro propagation makes industrial production of Cymbidium feasible.

In Vitro Propagation of Clivia

(1) Plant name Clivia grandiflora

In Vitro Propagation of Clivia

(1) Plant name Clivia grandiflora

(II) Material category embryo

(3) MS was used as basic culture medium. (1)callus induction medium: MS+2,4-D 1 mg/L + hydrolyzed protein 500;(2) bud differentiation medium: MS+BA2+ NAA 0.005 + hydrolyzed protein 500;(3) rooting medium: 1/2MS+ NAA 0.1 + KT 0.5 + LBA 0.5 + activated carbon 5g/L. All the above media were added with 3% sucrose, 0.8% agar, pH 5.8. Culture temperature (24 ± 2)℃, light requirements are not strict.

(iv) Growth and differentiation

1. Callus induction. Take the strictly sterilized peeled Clivia seed embryos and inoculate them onto the callus culture medium (1). After 20 days, radicle expanded, leaf primordia began to sprout, leaflets stopped growing soon, hypocotyls expanded, and yellow callus gradually formed, and then local callus turned green.

2. Differentiation and multiplication of buds. The green callus was transferred to differentiation medium (2) for further culture. The original green part gradually differentiated into small buds, and each callus could form 3-20 small buds. The callus of differentiated bud can be cut and subcultured continuously, so that a large number of test tube seedlings can be obtained.

3. Transplant. green seedlings with 2-3 leaves are cut and inoculated on the rooting medium (3), yellow conical root tips are generated at the lower incision after 1 month of culture, 1-5 different root tips are generated for each plant, and the root system is strong, the bottle stopper is opened, and the seedlings are cultivated for 3 days. After transplanting into Loropetalum chinense, do not let strong sunlight direct, temperature control in 20~C-25~C, air humidity maintained at about 85%, survival rate above 90%.

(5) Significance and progress Clivia is a perennial herb belonging to the genus Clivia of Amaryllidaceae. Clivia flowering as long as 3 months, but also when New Year's Day, Spring Festival. Lifetime green or dark green broad leaves, both sides of the development of uniform symmetry, has a very high ornamental value, economic value is also very high. At present, clivia propagation mostly adopts ramets, seed propagation, multiplication rate is not high, if the promotion of newly cultivated varieties, more slow, greatly limiting the production and development of clivia. In recent years, we have studied the rapid propagation of Clivia seed embryos in order to improve its rapid propagation coefficient. In vitro propagation of Clivia clivia initially solved the problems of slow growth and few tillers, and provided a feasible way for industrial production of Clivia clivia. A viable path.

(3) MS was used as basic culture medium. (1)callus induction medium: MS+2,4-D 1 mg/L + hydrolyzed protein 500;(2) bud differentiation medium: MS+BA2+ NAA 0.005 + hydrolyzed protein 500;(3) rooting medium: 1/2MS+ NAA 0.1 + KT 0.5 + LBA 0.5 + activated carbon 5g/L. All the above media were added with 3% sucrose, 0.8% agar, pH 5.8. Culture temperature (24 ± 2)℃, light requirements are not strict.

(iv) Growth and differentiation

1. Callus induction. Take the strictly sterilized peeled Clivia seed embryos and inoculate them onto the callus culture medium (1). After 20 days, radicle expanded, leaf primordia began to sprout, leaflets stopped growing soon, hypocotyls expanded, and yellow callus gradually formed, and then local callus turned green.

2. Differentiation and multiplication of buds. The green callus was transferred to differentiation medium (2) for further culture. The original green part gradually differentiated into small buds, and each callus could form 3-20 small buds. The callus of differentiated bud can be cut and subcultured continuously, so that a large number of test tube seedlings can be obtained.

3. Transplant. green seedlings with 2-3 leaves are cut and inoculated on the rooting medium (3), yellow conical root tips are generated at the lower incision after 1 month of culture, 1-5 different root tips are generated for each plant, and the root system is strong, the bottle stopper is opened, and the seedlings are cultivated for 3 days. After transplanting into Loropetalum chinense, do not let strong sunlight direct, temperature control in 20~C-25~C, air humidity maintained at about 85%, survival rate above 90%.

(5) Significance and progress Clivia is a perennial herb belonging to the genus Clivia of Amaryllidaceae. Clivia flowering as long as 3 months, but also when New Year's Day, Spring Festival. Lifetime green or dark green broad leaves, both sides of the development of uniform symmetry, has a very high ornamental value, economic value is also very high. At present, clivia propagation mostly adopts ramets, seed propagation, multiplication rate is not high, if the promotion of newly cultivated varieties, more slow, greatly limiting the production and development of clivia. In recent years, we have studied the rapid propagation of Clivia seed embryos in order to improve its rapid propagation coefficient. In vitro propagation of Clivia clivia initially solved the problems of slow growth and few tillers, and provided a feasible way for industrial production of Clivia clivia. A viable path.