How to improve the Survival rate of Edible Fungi in Summer

First, pay attention to the culture medium sterilization: the summer temperature is high, the preparation of the medium requires fresh, not mildew, mixed raw materials should be filled in time into bottles, bags sterilization. To make bottled bacteria, it is necessary to reduce the moisture of cotton plugs and Kraft paper as far as possible. Each time of sterilization, a small amount of cotton and Kraft paper are packed in plastic bags and sterilized together. After sterilization, replace the damp seal in the inoculation box in time. The use of bag-type production bacteria, each production link should be light, light handling, light handling, all punctured, crushed, dilated plastic bags, are sealed with medical adhesive tape or double-layer bags. The mouth of the bag can be sealed with rubber bands or tear bands instead of cotton plugs and rings. Depending on the amount of loading, the bagged substrate can appropriately extend the sterilization time by 1-2 hours, and the exhaust should be slow.

Second, improve the inoculation technology: due to the use of alcohol lamp flame inoculation in the inoculation box, the temperature often rises to 40-50 ℃, which is easy to burn or burn to death. Therefore, vaccination should be arranged in the morning and evening or when the temperature is slightly lower at night. During inoculation, the amount of each rotating bottle is less than that of conventional production, the amount of inoculation should be relatively increased, and the aged hyphae in the upper part of the bottle mouth should be dug up.

Third, strengthen the management of strain culture: the strain culture site is required to be clean, non-toxic, insect-free, cool, without sunlight, and the relative humidity is below 60%. The bacteria that have just been grafted must wait for the outside of the bottle bag and the seal to dry before stacking. The height of the stack and the distance between the piles should grasp the principles of not producing stack temperature, conducive to ventilation, convenient management and so on. The infection of seed production in summer is fast. Generally, it should be checked after 3 days of inoculation. Once the contaminated bacteria are found, they should be cleared in time.