Preparation of Liquid Strain of Edible Fungi

After installing the aeration pipe, oxygen was injected into the culture medium by oxygen supply machine.

The culture medium is placed on a shaker for shaking culture.

Edible mushroom liquid strain has the advantages of short production cycle, uniform age, convenient inoculation, fast fermentation, suitable for industrial production and so on. The production methods of edible mushroom liquid strains can be generally divided into electromagnetic stirring method, flask shaking machine method, simple submerged fermentation method and fermentation tank method. According to the staff of the edible fungus station of the Provincial Academy of Agricultural Sciences, the bottle shaking machine method is not only simple to inoculate and convenient to manage, but also the production capacity varies according to the setting of the machine, and the investment of 2000~3000 yuan can be made.

preparing liquid culture medium; extracting supernatant when potato is cooked but not rotten; preparing 3% glucose, 1% corn flour, 0.05% magnesium sulfate and 0.1% potassium dihydrogen phosphate; and finally mixing potato supernatant with the above solution and water. Liquid culture medium is suitable for culturing various edible fungi strains.

After the liquid culture medium of shaking flask is prepared, put it into Erlenmeyer flask with capacity of 500 ml, put 100 ml into each bottle, and add 1~15 small glass beads, seal the bottle mouth with cotton stopper and kraft paper, and sterilize it at 1.5 kg/cm2 pressure for 30 minutes. Then put a piece of slant strain of about 2 cm2, and culture it at 23℃~25℃ for 24 hours. When the hyphae germinate, the hyphae are placed on a reciprocating shaking table for shaking culture, the shaking frequency is 80 - 100 times per minute, and the amplitude is 6 - 10 cm. The temperature of the shaking chamber is controlled at 24℃~25℃, and the culture time varies according to different fungi, generally about 5 days. Pour the shaken culture medium into a large container, after expansion, install an aeration tube under sterile conditions, and culture for 48 hours after introducing proper oxygen by an oxygen machine. The standard of culture end: the culture liquid is clear and transparent, in which a large number of small mycelium balls are suspended, and accompanied by various mushroom unique fragrance. Cultured liquid strains should be placed in an incubator for preservation.