Understand how to cultivate edible fungi and the production points of edible fungi

In the production of edible fungi, if the environmental hygiene of the inoculation workshop is poor, the miscellaneous bacteria in the air will pollute the edible fungi, which will seriously affect the success rate of inoculation and the quality and output of edible fungi in the future. In the inoculation process, there are many miscellaneous bacteria that pollute edible fungi, including bacteria, molds, yeasts, actinomycetes and so on.

In the production of edible fungi, if the environmental hygiene of the inoculation workshop is poor, the miscellaneous bacteria in the air will pollute the edible fungi, which will seriously affect the success rate of inoculation and the quality and output of edible fungi in the future.

In the inoculation process, there are many miscellaneous bacteria that pollute edible fungi, including bacteria, molds, yeasts, actinomycetes and so on. The main reasons for microbial contamination of bacteria are as follows-

1. The strain culture room is dark and humid, the air is dirty, and there are many miscellaneous bacteria in the air, which pollutes the strains of edible fungi.

two。 The inoculation room is not strictly sterilized. The inoculation room was not sterilized effectively by using a suitable method.

3. The culture material was not sterilized thoroughly and was contaminated by miscellaneous bacteria. The main reasons for incomplete sterilization are: 1) improper sterilization. The mycelium and spores of general miscellaneous bacteria can be killed by sterilization methods of 100℃, 6-8h or 121℃, 0.5h-2h under normal pressure. At present, the culture materials of edible fungi are mostly sterilized under atmospheric pressure or high pressure. Atmospheric pressure sterilization is difficult to sterilize grain, dung grass and other materials, and it is not easy to achieve the effect of complete sterilization, while the effect of high pressure sterilization is better. 2) different culture materials. The sterilization effect is affected by the kinds of materials needed for producing bacteria, the bacterial content of culture materials, water content, pH and other factors. The corresponding sterilization time should be selected according to the characteristics of different culture materials. 3) the influence of strain packaging container. In seed production, the bacteria are usually packaged in bottles or bags. The heat transfer rate of plastic bags is faster, while that of glass bottles is slower. When the same culture material and the same sterilization method are used, the sterilization time of bottled material is longer than that of bagged material.

4. There are miscellaneous bacteria in the bacteria. The original species of edible fungi used for expanded culture must be pure bacteria in order to ensure the singleness of the strains. If the edible fungus contains miscellaneous bacteria, it will lead to a decrease in the vaccination rate.

5. The vaccination personnel operated improperly. The inoculation process of edible fungi requires a strict aseptic operating environment. If the operation is not standard, it is easy to cause the culture material to be contaminated by miscellaneous bacteria. The miscellaneous bacteria that pollute the culture material are generally the miscellaneous bacteria in the air of the inoculation room, the miscellaneous bacteria on the surface of the bacteria bag and the miscellaneous bacteria on the body surface of the inoculators.