Notes on introduction of Edible Fungi and simple Preservation of Mother species of Edible Fungi

The mycelium of edible fungi propagates rapidly and is easy to mutate, so it is difficult to preserve the strains. In this paper, several effective and simple methods of strain preservation are introduced.

1. Liquid paraffin preservation method: select chemical liquid paraffin, put it in a 40 ℃ thermostat for hours or days after high pressure sterilization, absorb liquid paraffin with a sterile straw or syringe, and inject it into the strain test tube. The injection volume should be about 1 cm higher than the tip of the slope, plug with cotton plugs, seal with wax, store upright in low temperature, and can be preserved for 5 years.

2. Wheat bran preservation method: add a certain amount of wheat bran to water or nutrient solution and mix well. The water and wheat bran were loaded into the test tube, sterilized under high pressure and then cultured at the appropriate temperature. After the mycelium is well developed, the test tube is put into the drying chamber for drying. Store it in a dry place below 20 ℃ after drying, which can be preserved for 3 to 5 years.

3. Physiological saline preservation method: put the bacteria into the potato culture medium, 60 ml culture medium in 250 ml triangle bottle, shake or shake 5 times a day for 5 times a day for 5 days. Then transfer the formed mycelium balls into a test tube containing 5 ml of sterile normal saline, insert 4-5 mycelium balls in each tube, plug them with cotton plugs, seal the tube mouth with wax, and store them at low temperature for 1 year.

4. Wheat grain preservation method: take high-quality wheat, soak it in 20 ℃ water for 5 hours, dry it a little, and then load it into a test tube. After sterilization, shake while hot, cool into the mycelium, culture at suitable temperature until sparse hyphae appear on most wheat grains. It can be stored in a dry place below 25 ℃ for 1 to 2 years.