Matters needing attention in rehydration of edible fungi, four methods to identify the quality of edible fungi

1. Direct observation and identification of appearance

Appearance bacteria, mycelium thick white, stout, elastic, then strong vitality; if the mycelium atrophy, dry no color, or mycelium autolysis produced a large amount of reddish-brown liquid, then the vitality has become weak, not suitable for re-use; if the wood block fungus still remains hard, it belongs to the strong vitality strain, if the wood block becomes softened and loose, it has been aged and should not be used.

Second, culture, observation and identification

For the isolated, selected and introduced strains, the adaptation characteristics of mycelium to dryness, humidity and temperature were observed through culture. If the mycelium is cultured under dry, wet and dry-wet conditions, it is a good strain if the mycelium can grow well under the first two conditions and the best under the condition of suitable dry and wet.

3. Identification of liquid culture

Prepare 2% sugar solution, often sterilize and disinfect, pick up the bacteria with the size of soybean grains, put them in 100ml of the above solution and culture them at 25 ℃ for 7 days, if the liquid surface appears bubbles, oil skins and turbidity, it indicates that the bacteria itself has miscellaneous bacteria; if the seedlings sink or grow a very thin mycelium layer, it means that the strain is weak in life. If the mycelium around the liquid surface grows fast and is thick and white in the shape of cotton floc, it shows that the strain has strong vitality.

IV. Identification of sawdust bottle cultivation and periodic yield

The method of bottle identification is similar to the method of cultivation and cultivation. The sawdust culture material is loosened, the humidity is increased appropriately, the bacteria that need to be identified are inoculated in the culture medium, and the record is made. The sawdust culture material is cultured at 26 ℃ for 15 days. Then the temperature was lowered to 15 ℃ and 20 min, and better scattered light conditions were given. After about half a month of cultivation, fruiting body primordia and a small amount of fruiting bodies will appear on the bottle wall and material surface. If no miscellaneous bacteria and abnormal phenomena are found, the bacteria are connected to the wood section and the periodic yield is identified. If the fruiting body has exuberant growth, high yield and high quality, excellent variety characteristics, and no mixed bacteria, it shows that the strain is reliable and can be preserved and put into large-scale production.