Cultivation techniques of out-of-season Lentinus edodes

1) Preparation. Raw materials used corncob, sawdust, cottonseed hull, accessories used rice bran or wheat bran. Before the greenhouse is used, 15 grams of sulfur is used per cubic meter. After ignition, sulfur dioxide can be produced. It has strong sterilization power and is used to disinfect the greenhouse. When sulfur is used to fumigate the greenhouse, the doors and windows should be closed tightly and people should leave to avoid poisoning. 3% Lysol and Bordeaux can also be used to disinfect greenhouses. Before fruiting, the shed should be dry, and the relative humidity of the air should be maintained at about 70%. Moisture is easy to cause miscellaneous bacteria infection. In order to make full use of the space, the shed can be built, each layer of 30 cm, can be built 5 layers, set aside sidewalks between the shelves. You can also stack the bacteria directly on the ground without erecting a shelf, which can be stacked 1 meter high.

2. Prepare culture medium. Formula: corncob or hardwood sawdust, cottonseed hull 80%, fine rice chaff or wheat bran 18%(only one kind), sugar and gypsum 1% each. To prevent mold infection, add 1 kg of carbendazim per 1000 kg of feed. Corncobs are ground to bean-sized pieces. Weigh and mix the ingredients according to the proportion and add water to make the water content reach 60%, that is, hold the ingredients with hands and fingers without dripping, pH 5.5-6.0.

3. Bagging. Inoculation room with sulfur fumigation or spray lysol disinfection in advance. Vaccination personnel wear clean clothes, wash hands, and wipe hands with 75% alcohol cotton wool. All utensils should be boiled or immersed in 0.5% potassium permanganate solution for disinfection. Prepare plastic bags 17-30 cm in diameter and 50 cm long. Mix the culture material according to the proportion, add water and mix well, and bag immediately. Each bag is about 5 kg, moved into the inoculation room, or bagged and inoculated immediately.

4. Vaccination. Pay attention to aseptic operation when inoculating, wear mask, wear white hat, cover hair, wash hands with soap, and wipe hands with 75% alcohol cotton. There are two ways to vaccinate. One is stratified inoculation, which is carried out simultaneously when bagging. First tie one end of the bag, pour 1-2 bottles of bacteria, then fill, fill half the bag and then pour 2 bottles of bacteria, fill the bag and then pour 1-2 bottles of bacteria, and then tie the mouth. 0.5-1 kg seed per bag. Another method of inoculation is punch inoculation, in which 6 holes are punched in each bag. Make a 1.5 cm thick small stick, the top of the stick is flat, aim at the plastic bag filled with materials and poke hard to make a hole, put the strain into the hole by hand, inoculate 100- 200g in each hole. Inoculated holes do not need to be sealed with adhesive tape, can be exposed, conducive to ventilation and oxygen.

5. Management of hypha growth period. The inoculated bags are stacked together, up to 1 meter high, leaving sidewalks. The bag stack is covered with a layer of kraft paper to block light and keep warm. The shelf shed can be placed separately, and the same kraft paper can be covered. Open the straw washi quilt on the roof in time in the morning and cover it again at night. Mycelial growth stage, shed temperature maintained at 5-15℃, with low temperature culture hyphae, not more than 20℃, or a large number of miscellaneous bacteria occur. Shed water and spray water can not be kept dry, air relative humidity to maintain about 70%. When the mycelium on both sides of the bag has reached 5 cm, the two ends of the bag can be loosened to allow it to enter the air and increase ventilation and oxygen supply. 40-45 The hypha grows full of bags, and begins to change color, from white to brown, the whole fungus block becomes one. At this time, the bag openings at both ends can be completely opened, and ventilation can be increased. The color conversion period is 10-15 days.

6. Management of fruiting period. After the whole fungus block turns yellow brown and exudes some yellow brown water, it is managed in the fruiting period. At this time to improve the humidity in the shed, the ground can be sprinkled, the air with a sprayer spray, so that the relative humidity of the air up to 90%. Increase the transparency appropriately, but still do not let sunlight shine on the bacteria block. The daytime temperature is maintained at 15-20 ° C and the night temperature is 5-10 ° C, resulting in a certain temperature difference. If placed separately, the bag can be removed. If it cannot be placed separately, it can still be stacked together. It is not necessary to take off the bag. Only roll up the bag openings at both ends, and carry out simple grading. Cut off one section of mushroom stem and transport it to the market for sale in time. Do not store it for a long time to avoid the loss of rotten mushrooms. If it cannot be sold immediately, it can be dried, dried or salted in time.