Virus-free technology of potato

Potatoes are nutritious and resistant to storage. It has a short growth period and great potential to increase production, so it is fond of eating for people. However, potatoes are easy to be infected with a variety of viruses, resulting in smaller tubers, deformities, seed potato degradation and so on. The experimental results show that the detoxification of potato by stem tip tissue culture combined with virus detection and the production of virus-free seed potato can effectively prevent the degradation of seed potato and greatly increase the yield of potato. The main detoxification methods are as follows:

1. Take materials and disinfect the tubers to sprout. When the buds grow 4~5cm, cut the buds and peel off the outer leaves, rinse 40min under tap water, disinfect with bleach solution in the sterile room, rinse with aseptic water for 2 times.

2. Peel and inoculate in the sterile room, under 40 times dissection microscope, peel off the stem tip of the original base with one leaf, and inoculate it in the test tube of MS stem tip culture base. The MS shoot tip medium in the test tube consists of a large number of elements, trace elements, organic components and auxin, cytokinin, sucrose and Agar. The pH value is 5.7. after high pressure sterilization, one stem tip is inoculated in each test tube.

3. Under culture conditions, the shoot tips inoculated were cultured in the room under 25C and 1500~3000LX light, and grew into plantlets with 3-4 leaves in 3 months. Under aseptic condition, cut and propagate once, and some seedlings were taken for virus detection.

4. virus detection is an indispensable step for stem tip detoxification, which is often detected by differential hosts, that is, indicator plants or serological methods, to eliminate the stem tip seedlings with positive serological reaction or symptoms on the indicator plants in time. The stem tip seedlings without any reaction are used for reproduction.