Technical specification for feeding management and artificial insemination of breeder Roosters

Breeding, management and insemination of Roosters

The artificial insemination technology of chicken has been widely promoted in many large-scale breeding enterprises, especially the artificial insemination of breeder chicken is a serious, meticulous and highly technical work, including breeding rooster selection and semen quality examination. the operation techniques of artificial semen collection and insemination should be grasped accurately.

This paper is a set of operation rules of artificial insemination technology summed up by combining the breeding process of the company's new varieties "Jinghong No. 1" and "Jingfen No. 1" and refining the key technologies of production. This procedure plays a key role in improving the fertilization rate of breeder chickens.

1. Breeding and management of Roosters

1. Selection criteria of breeder Roosters

①, appearance

Strong physique, strong muscles, broad chest, bright eyes, agile, clear voice; strong legs and feet, strong cushions; plump and glossy feathers, no miscellaneous colors, obvious secondary sexual characteristics, good development of chicken crown and beard, bright red color is the best.

②, production performance

Semen collection is generally 0.4-1mL, semen sticky, milky white. If possible, the density and motility of sperm can be monitored by electron microscope for further screening. The density of normal chicken is generally 25-4 billion / mL, and the sperm moves in a straight line without deformity.

Key points of breeding ③ and breeder Roosters

The first time: at the age of 1 day, the weak rooster should be eliminated appropriately according to the ratio of male to female during the laying period.

The second time: about 40 days old, choose the rooster with good development and bright red crown.

The third time: 17-19 weeks old, choose a rooster with good sexual characteristics, strong physique and sexual reflex.

The fourth time: at the age of about 22 weeks, the rooster with no semen or poor semen quality was eliminated during semen training.

2. Training methods of breeder Roosters

①, training time

Between 145 and 154 days of age, once every two days, generally training for four times until insemination.

②, training methods

The speed of holding the chicken should be light and fast, hold the root of the rooster's legs slightly downward with the left hand, and pay attention not to exert too much force. the body of the rooster is parallel to the left arm of the person holding the chicken, so as to make it in a natural state as far as possible. the semen collector uses back massage to caress 2-3 times from the root of the wing to the tail, and then gently pinch both sides of the cloacal cavity and gently shake the index finger and thumb to massage.

2. Technical essentials of artificial semen collection of breeder Roosters

1. Preparation before semen collection

The instruments needed in the semen collection process should be fumigated and disinfected before semen collection, and the seminal duct should be preheated (winter: 36-37 ℃, summer: 35-36 ℃). Roosters who collect semen should stop eating for 3-4 hours before semen collection.

2. Matters needing attention in semen collection

The action of ① and semen collection should be light, so as to avoid sperm damage caused by human factors.

In the process of ② and semen collection, personnel are required to cooperate skillfully to prevent each other from waiting for each other, resulting in prolonged sperm collection time or reduced sperm collection.

③, if blood or semen is found to be thin during semen collection, the rooster should be picked out and suspended for use; if feces, feather shavings or other dirt are collected carelessly, semen should be discarded.

Don't use too much force in the mixing process.

3. Semen quality evaluation

The quality of semen, including semen density and vitality, should be examined by electron microscope regularly, and the rooster with poor semen quality should be eliminated in time so as not to affect the artificial insemination of breeder chickens.

III. The essentials of turning the anus in artificial insemination

1. Master the essentials of turning the anus

Anal turnover personnel in the operation should be light, light, accurate, stable, fast, not rough, to prevent the eggs in the fallopian tube from being crushed, resulting in salpingitis or peritonitis. When you put pressure on the hen's abdomen when turning the anus, you must focus on the left side of the abdomen, especially when the tubal orifice just protrudes the cloacal cavity.

2. Prevention of human infection

In order to avoid the occurrence of tubal inflammation caused by artificial infection in the process of turning anus, the hands of the operation of turning anus were wiped and disinfected with a towel stained with disinfectant before each turn.

IV. Key points of artificial insemination

1. Prevention and treatment of cross-infection

During insemination, one chicken should be insisted on using a drip head to reduce the cross-infection of diseases among chickens.

2. Key points of insemination technology.

The insemination personnel inserted the vas deferens vertically along the center of the oviduct orifice to prevent the fallopian tube from hitting the side wall of the fallopian tube, causing fallopian tube damage. The depth of insemination was generally "Jinghong No.1" 3.0cm, "Jingfen No.1" 1.5-2.0cm, and the insemination volume was "Jinghong No.1" 0.025mL, "Jingfen No.1" 0.02-0.025mL. When pulling out the vas deferens, the emitter should not carry semen. If there is semen, it should be infused repeatedly. The temperature difference of the heat preservation cup before and after insemination should not be higher than 1-2 ℃.

3. Points for attention

①, the process of deferment, the vas deferens can not be with air bubbles or air column, but also not with feather shavings, feces, blood and other sundries.

②, from semen collection to full use of semen, that is, the time of insemination should not exceed 30 minutes in principle.

③, minimize the exposure time of fallopian tubes to the outside world, and avoid waiting for people to turn the anus after semen aspiration.

④, chicken flipping personnel catch the chicken gently as far as possible to minimize the chicken stress.

⑤, when absorbing semen, you should try to absorb it on the surface of the semen level to avoid inserting the eyedropper into the deep part of the semen.

After ⑥ and insemination, the flip chicken personnel must see whether the semen is taken out, and the outflow is replenished. At the same time, do not push the chicken abdomen to prevent abdominal pressure and semen outflow.

To improve the rate of artificial insemination of chickens, we should pay attention to the "seven hurdles".

The first is to keep good breeders. The breeder chickens of artificial insemination are raised in cage. As the range of movement in the cage is small, it is easy to cause fat deposition, so we must strictly limit the amount of feeding according to the weight gain of chickens to prevent the formation of fatty liver and prolapse of the anus, otherwise it is not conducive to semen collection of Roosters and anal insemination of hens.

The second is to check the selection and training of good breeding Roosters. The breeder rooster used for artificial insemination should not only conform to the breed characteristics, production performance and good health condition, but also choose the rooster with soft abdomen, upward tilting of the tail when lifting the wings, anal eversion during massage, large and loose cloacal cavity, moist, large copulator and erection. Roosters with good quality of ovulation. Two weeks before insemination, the proposed rooster should be trained to form a good conditioned reflex to Baoding, massage and ejaculation, so as to understand the sexual reflex habits of each rooster. Training usually takes about 7 times.

Third, do a good job of disinfection. All appliances in contact with artificial insemination must be strictly and thoroughly cleaned and disinfected before use. The most convenient and effective disinfection method for chicken breeders is to boil, and the water used must be distilled water. Dry the moisture after disinfection. Utensils and hands of operators that cannot be sterilized by boiling can be sterilized with alcohol. Do not operate until the alcohol is fully evaporated.

The fourth is to check the quality of the good ones. Semen collection should be carried out 3-5 hours after the water is cut off. Semen collection should be based on the sexual reflex habits of each rooster, grasp the appropriate number of massages and the size of the force. If the massage time is too long or too hard, the rooster will form reverse stimulation without sexual reflex, or contaminate semen because of defecation, urine and mucous membrane injury; if the force is too small, the extroversion of the copulator is not sufficient, it is easy to retract. When collecting semen, the fingers and the collecting cup are not allowed to touch the copulator. A rooster should use a collecting cup, and then combine the semen with an eyedropper after harvest, so as to prevent unclean semen from affecting the quality of the whole cup, and semen contaminated by feces should be abandoned. When collecting semen in winter, the collector cup should be preheated to 35 ℃ ~ 40 ℃.

The fifth is to ensure the quality of semen. The high quality semen is milky white and thick liquid. When the breeder chicken is sick or the semen is mixed with feces and urine, the semen is often abnormal and thin, and this semen is not suitable for insemination. The motility and density of sperm should be examined under microscope.

The sixth is to dilute the semen. Dilution should be carried out immediately after semen is collected. When preparing the diluent, the utensils should be clean, the weighing should be accurate, and the medicine should be pure and fully dissolved. The dilution multiple should be calculated accurately before dilution, and the diluent should be preheated to 35 ℃ ~ 40 ℃. When diluted, it is slowly added to the semen along the wall of the collector cup, and finally mixed with an eyedropper.

The seventh is to keep good insemination. During insemination, the action of inserting and sucking mouth should be light, not hard, to prevent vaginal injury. The suction mouth should be inserted into the vagina 2.5 to 3 centimeters deep. Do not relax the knob immediately after semen pressure out, so as not to add semen; the suction nozzle should be drawn out against the upper wall of the vagina, so as not to cause semen outflow. Semen infusion to ensure that there are 10 million effective sperm per day, usually requires the infusion of good appearance quality of 50 microliters of original semen. Insemination should be carried out 3 hours after laying eggs. Under normal circumstances, the production of chickens can generally be arranged for 6-7 days as a semen cycle.