Two Key Control Chains in Porcine Artificial Fertilization

In recent years, pig artificial insemination technology has developed rapidly, large-scale pig farms have basically carried out artificial insemination work, and there have been some large-scale boar breeding stations (companies) mainly selling boar semen, mainly including Beijing@#@227@#@Breeding Center affiliated to the Ministry of Agriculture North SPF boar breeding station and Beijing Haobang pig artificial insemination service limited liability company. At the same time, in rural areas where individual pig farmers are concentrated, small boar stations with 3 to 5 boars invested by individual farmers appear like bamboo shoots. The author summarized the working experience in SPF boar breeding station of Beijing Pig Breeding Center and summarized two key control chains of pig artificial insemination.

The two key control chains in artificial insemination are cleaning chain and temperature chain, which run through every link of artificial insemination. The main contents of cleaning chain include management of breeding boar station, management of semen collection room and semen collection personnel, management of semen dilution in laboratory, sperm infusion procedure, etc. The main contents of temperature chain include preparation before semen collection, semen dilution, semen subpackage, semen preservation and transportation, etc.

cleaning chain

Breeding Management of Breeding Boars

Clean the pig house regularly every day, train the breeding boar to eat, sleep and excrete three points, so that the breeding boar maintains a good living environment. Keep the food trough clean and clean it with high-pressure cleaner at least once a week; check the quality of feed before feeding each time, destroy moldy and deteriorated feed found, and do not feed breeding boars; clean the feces and urine in the pigpen in time to reduce the chance of boar body and foreskin contamination; brush the boar body regularly to remove dust and dander from the breeding boar body; Disinfect the pigsty twice a week. Use Guangpu high-efficiency disinfection drugs such as Baijunxiao-30, Miduwei, Disinfectant, Peracetic Acid, etc., prepare according to the disinfection concentration specified in the instructions for use. Mix evenly before disinfection. The dosage of disinfectant shall be 0.3L/m2. Replace the disinfectant regularly.

Management of semen collection room and personnel

The sperm collection room should be clean and dry, and there should be no foreign matter on the ground. The ceiling of sperm collection room adopts aluminum gusset plate or plastic steel plate to reduce dust falling on it, and it is cleaned once a week. Sperm collection personnel wear sanitary hats to prevent hair and dander from falling off and contaminating semen. Before collecting semen, squeeze out foreskin urine in time, clean foreskin with toilet paper and wipe foreskin thoroughly. Sperm collectors must wear gloves when collecting semen to prevent cross-contamination of semen. At the same time, sperm collectors must regularly trim their nails to prevent excessive fingernails from damaging gloves and contaminating semen. During semen collection, do not collect the first semen and the last semen. The first semen may be mixed with a small amount of urine, and the last semen contains more jelly and less sperm, so it is not suitable to collect. After semen collection, tie the bag and cover the thermos cup immediately.

Management of Semen Dilution in Laboratory

Clean the laboratory regularly every day to minimize dust on the floor, countertops, walls and ceilings of the laboratory. Sperm dilution personnel must change their overalls and shoes and hats when entering the laboratory. After each use, the collection cup, dilution cup, glass rod and dilution powder bottle should be thoroughly cleaned, rinsed twice with double distilled water, and then sterilized by high pressure or dry baking (depending on the nature of the instrument). Semen dilution must be performed with double distilled water or deionized water, and the shelf life of double distilled water and deionized water cannot exceed 1 month.

semen infusion procedure

Inseminating process attention clean, reduces the womb inflammation occurrence. Estrus boar is placed in front of oestrus sow to stimulate sow. Clean the vulva, tail root and arm area of sow with 0.1% potassium permanganate solution, wipe the vulva area of sow with clean toilet paper. Gently mix the semen so that it is evenly distributed in the vial and insert the vas deferens into the sow's vagina. Attention to the above details is one of the reliable guarantees that the sow uterus is not contaminated.

temperature chain

Preparation before semen collection

Before semen collection, all articles in contact with semen, including gloves, semen collection cups, semen sub-packaging bottles, etc., should be preheated at 37℃ in an incubator to ensure that the temperature difference between semen and articles in contact during semen collection is not higher than 2℃. Semen dilution should also be preheated in a 37℃ water bath at least 1 hour in advance to ensure that the diluent is evenly mixed. 25℃ is the most suitable setting for laboratory air conditioning.

semen dilution

After semen collection, semen quality test should be carried out immediately, including sperm color, smell, vitality and density, etc. Finally, semen dilution should be carried out according to the test results. The temperature difference between diluent and original sperm should not be higher than 2℃, otherwise it will seriously affect the sperm motility after semen dilution.

Subpackaging, Preservation and Transportation of Semen

After semen dilution, sub-package immediately. Take out the semen sub-package bottle from the incubator, so that the temperature difference between diluted semen and semen sub-package bottle is not higher than 2℃. After semen sub-packaging, it is not advisable to immediately put semen into an incubator at 17℃ for preservation. After allowing semen to slowly cool at room temperature of 25℃, put it into an incubator. During semen transportation, an incubator should be used to ensure that sperm motility is not affected.