Development and utilization of new functional components of soybean

China is the hometown of soybeans. In the long history of development, "soybeans have nurtured the Chinese nation". Modern science has found that the functional components of soybean that nurture the Chinese nation mainly exist in soybean meal, and high-temperature soybean meal is the product with the highest output in soybean oil processing industry. Whether the effective components in high-temperature soybean meal can be extracted is to revitalize China's soybean oil processing industry.

Invented by the National Soybean Deep processing Technology Research and Promotion Center, high-temperature soybean meal is used as raw material to continuously extract high-purity soybean oligopeptides, soybean isoflavones, soybean compound efficacy factors and special soybean protein concentrate for noodle products. because the production process and physical and chemical indexes of the extract are different from similar products at home and abroad, it belongs to independent innovation @ # @ 78 efficacy ingredients, so it is temporarily called "new efficacy ingredient".

1. New efficacy components and efficacy Test of Soybean

The main results are as follows: (1) since the 1970s, it has been found that soybean protein can be hydrolyzed and refined to produce a small molecular protein, which is different from the common protein with complex structure and molecular weight above 105D. The industry standard of soybean peptide powder was established in China at the beginning of the 21st century, and the soybean proteins with molecular weight no more than 104D were collectively referred to as soybean peptides.

The high purity soybean oligopeptide is produced by this project: the high purity soybean oligopeptide is used as raw material to separate and extract non-protein components for the first time, reverse retention and biological enzymatic hydrolysis of insoluble protein. The protein purity of the product is not less than 96.02%. The composition of the product is much higher than that of similar products at home and abroad, and the molecular weight distribution is not more than 1041), which is obviously smaller than that of similar products at home and abroad, with solubility of 100% and NSI value of 100%. The ash content is not more than 1.5%. In order to distinguish from similar soybean peptide products at home and abroad, it is temporarily named "high purity soybean oligopeptide". The comprehensive cost of high purity soybean oligopeptide production is 50 yuan / kg, and the ex-factory price is 150 yuan / kg. Every ton of soybean meal only processes positive high purity soybean oligopeptide, and the profit and tax is more than 10 thousand yuan.

The results showed that after feeding pigs with high purity soybean oligopeptide, it was detected in blood after administration of 5min, and the blood concentration of drug reached the peak after administration of 10min. High-purity soybean oligopeptides can be quickly absorbed into the blood without digestion and enzymolysis after ingestion, which is about 2% of the blood entry time rate that can be detected in the blood after the intake of ordinary protein.

High purity soybean oligopeptide was given to Kunming mice by intragastric administration of 10g/kg~30g/kg. The results of acute toxicity test showed that there was no poisoning in mice.

After taking high purity soybean oligopeptide per 1kg body weight, the swimming time of the experimental group was significantly longer than that of the control group. The longest swimming time of the experimental group was 10min and 20min, and the average swimming time was 4.12times and 5.32times of that of the control group, respectively. It is completely consistent with the results of pharmacokinetic test, that is, after the intake of high-purity soybean oligopeptide, 10min reaches the peak value of blood medicine, at the same time, it produces obvious rapid transformation of physical energy, which can produce the effect of anti-fatigue in a short time (10min).

(2) the high purity isoflavones extracted from soybean isoflavones in this project, the total isoflavones are 95.93%, of which genistein is 90.61% and daidzein is 5.32%. The products in the pilot stage have been exported to Japan.

Genistein and daidzein belong to the same family of soybean isoflavones, but their medical and health effects are different because of their different structures.

The experimental results showed that high purity soybean isoflavone had obvious inhibitory effect on mouse breast cancer, and the tumor inhibition rate was 36.0%-61.3%. At the same time, it shows that women taking low-purity soybean isoflavones is about 5g per day, which is equivalent to taking genistein (G) is about 20mg. The difference is significant by self-control statistical treatment, which can significantly increase the level of female estradiol and delay menopause and aging.

(3) Soybean compound efficacy factor is a new product of this invention, which contains a variety of nutrients necessary for human growth and development and medical care.

The soybean compound efficacy factor produced in this project has low production cost board due to comprehensive processing, and the comprehensive production cost per 1kg is 30 yuan. If the daily intake of 20mg~90mg isoflavones per person recommended by FDA in the United States is used for the prevention and treatment of female tumors, the daily drug expenditure is only 0.09 yuan ~ 0.15 yuan. It is a cheap new product that can realize the popularization of health care and nutrition.

(4) the special functional soybean protein concentrate for flour products produced by this project, due to the significant reduction in the cost of comprehensive utilization, the cost is less than 3000 yuan / t, and the export price of the pilot product is US $5500.It is 50% of the current selling price of the same kind of protein concentrate (protein ≥ 65%) in our country. It can strengthen the protein content of flour.

In November 2000, the American Heart Association pointed out that the intake of 50g soy protein per person per day can effectively reduce the harm of heart disease to the human body. The test results showed that compared with the control flour, the quality of high protein flour with functional soybean protein concentrate for flour products was improved comprehensively, including 10% increase in cake, 2% increase in bread and 3% increase in noodles.

The addition of functional soybean protein concentrate to flour products to strengthen the nutrition of soybean protein, a staple food of flour, is the simplest, safest and most convenient way to increase soybean protein intake by the whole people in the National Soybean Action Plan. Because high-protein flour basically does not increase the economic burden of the country and residents, it can also improve the nutritional supply of soybean protein and effectively prevent and cure cardiovascular diseases. In 2004, the high-protein flour successfully developed by this invention has been approved as "National Science and Technology achievements key Promotion Plan Project" (2004EC112) by the Ministry of Science and Technology, and the patented product (ZL99113106.1) high-protein flour developed by this project in 2005 has been approved as "Nutrition and Health Promotion Product" by the Public Nutrition and Development Center.

two。 Benefit Analysis of New functional components of Soybean

In the field of soybean processing, it is believed at home and abroad that irreversible excessive thermal denaturation makes high-temperature soybean meal lose its processing function and can only be used as feed. The independent innovation invention patent of this project adopts separation and extraction of non-protein components in high-temperature soybean meal, reverse retention and biological enzymatic hydrolysis of over-denatured protein, and comprehensively extracts all the beneficial components in high-temperature soybean meal to human beings. the COD value of production discharge water is not higher than 25mg/L, and the cost is greatly reduced. Due to the separate extraction, the adverse effect of reducing the purity caused by the mixing of different components is avoided, thus the purity of the product is significantly improved. Due to continuous extraction, the variety of products is higher than the production quantity of other production processes, and due to the improvement of comprehensive yield, the comprehensive benefit per unit of raw material soybean meal processed by this invention patent technology has been greatly improved compared with the same period last year. Taking the processing of 1t high temperature soybean meal as an example, the profit and tax can be increased by more than 20,000 yuan. Even if the benefit fluctuates, according to the fluctuation coefficient of 50%, the processing profit and tax for every 1 ton of soybean meal is more than 10,000 yuan, and the new efficacy ingredients of soybean produced by this project do not have any toxic and side effects. At present, when all mankind generally pursue nature, health, beauty and longevity, the new efficacy ingredients of soybean will have a broad market. The high-purity and low-cost soybean processed products produced by this project will play a positive role in promoting the overall revitalization of China's soybean processing industry and increasing foreign exchange in the face of the world.

On the premise of not affecting the demand for high temperature soybean meal in China's feed industry, the deep processing of China's resource-rich high temperature soybean meal by self-created high technology can greatly increase the added value and shorten the period of turning losses into profits in the oil processing industry. promote the development of soybean industry.

The methods of extracting protein, short peptides, nucleic acids, isoflavones, saponins and oligosaccharides from high and low temperature soybean meal and the method of extracting compound soybean functional factors from high or low temperature soybean meal have the characteristics of high added value. soybean processing is the leader of the development of soybean industry, processing industry produces high benefits, high-benefit stimulation will inevitably lead to the great development of soybean industry. The planting of raw material soybean is the first workshop of soybean processing. after the transformation according to the contents of the national key promotion plan for scientific and technological achievements, the soybean processing industry that adopts the patent of the invention should carry out order agriculture to the specialized households of raw material soybean production in the form of orders. the soybean processing industry will increase the profits of the soybean processing industry in the way of high quality, high price and industry to feed agriculture, and appropriately give benefit to the professional households of raw material soybean production, although soybean processing enterprises give profits to soybean farmers. However, because the patented technology of independent innovation and invention has high added value, it does not constitute an additional burden for processing enterprises, but a significant increase in income for farmers, which is equivalent to increasing soybean farmers' income without increasing arable land or agricultural investment. rely on independent high technology to increase soybean farmers' income, so that soybean processing enterprises and raw soybean farmers to achieve a win-win situation. Independent innovation of soybean processing high technology will have a positive practical effect on farmers getting rich quickly and shortening the cycle of solving the problems of agriculture, rural areas and farmers in our country.