The reproduction method of summer pink

Carnation is a perennial perennial herb with swollen nodes, opposite leaves, l0-15cm in height, evergreen in four seasons and flowering in three seasons. It has wide adaptability, cold resistance (low temperature of minus 35 ℃), drought and barren resistance (watering only once a month, little fertilization), beautiful flowers (flowers can cover the ground in full bloom), and not very strict requirements for soil acidity and alkalinity (soils with pH values between 5 and 8 can be planted), strong ability of division and differentiation, easy reproduction and rapid growth.

The root system of carnation in summer is huge, which can prevent soil erosion and soil erosion, and can resist harmful gases, diseases and insect pests, and has important functions of health and environmental protection. As an important part of the garden, it can also be cultivated inside and outside the courtyard, on both sides of the highway, scenic areas, as well as open-air activities and rest areas, which not only has a good role of beautification and greening, but also has a strong ornamental.

At present, the common lawns are Carex angustifolia, Bermudagrass, carpet grass and so on. Bright green in spring, summer and autumn, withered and yellow in winter, no flowering, lack of cold resistance, stress resistance, beauty and persistence. If combined with the characteristics of local resources and market advantages, carnation is introduced to form large-scale production, and the existing inferior lawns are gradually renewed, which can produce greater social benefits. at the same time, according to the current market price of 1.10 yuan per tree, 11 seedlings per square meter are needed, and the economic income of 6000 million yuan per mu will be obtained, and the economic benefit will be considerable.

At present, the main propagation methods of Phyllostachys pubescens are ramet and tiller, but it is difficult to propagate a large number of seedlings quickly, which is one of the important reasons why carnation seedlings are expensive at present. This paper introduces a method of rapid propagation of test-tube plantlets by tissue culture of terminal buds and axillary buds of Phyllostachys pubescens in Changxia. Using this technique, millions of seedlings can be propagated in a short time, which has the characteristics of low cost and high benefit.

First, prepare the culture medium

According to different needs, all kinds of chemical reagents were weighed strictly and accurately and prepared into different types of culture media. Callus induction medium was MS+2mg/L6-BA; differentiation medium, MS+lmg/L6-BA+0.1mg/LNAA; rooting medium was MS+0.1mg/LIAA+0.Img/LIBA. The high pressure sterilization condition is 120 ℃, 1.1 Mel 1.2 atmospheric pressure, 20 minutes.

Second, training methods

1. Callus culture. Wash the young branches of Phyllostachys pubescens in clean water, cut off the leaves and most of the stem segments, take the stem tip and axillary buds, rinse for 2 times and drain. On the ultra-clean worktable, soak the stem tip with 75% ethanol for 30ml for 60 seconds, then soak it with 0.1%hgcl2 for 10 minutes, and finally rinse with sterile water several times. The tissue fragments including the growing points were removed with tweezers and scalpel and inoculated on the callus induction medium. The culture conditions are as follows: the light intensity is 1000-2000Lx, the temperature is about 25 ℃, and the light intensity is 10ml / day for 12 hours. After about 15 mi for 25 days, the inoculated explants could expand and form callus. After 1 week, the callus could be subcultured or differentiated.

two。 Subculture. The callus was removed from the test tube or culture bottle under aseptic condition, and the medium attached to the callus was removed with tweezers and scalpel, and washed repeatedly with sterile water until there was no residual medium on the callus. The callus was cut into several small pieces with scalpel, and then inoculated into the culture flask with callus induction medium, and continued to culture under the same culture conditions as callus induction. After a few days, the small callus in the culture bottle can grow up gradually, and this process can be operated repeatedly. Through the subculture of callus, a large number of new branches could be produced from a shoot tip tissue inoculated originally, which could be used for differentiation culture.

3. Differentiation and culture. Under the aseptic condition, the callus was removed from the test tube or culture bottle, washed with aseptic water, cut into small inoculation pieces, and inoculated into the culture bottle with differentiation medium. After about 1 month, the callus can differentiate into buds and continue to grow new branches. When the new branches grow to 1-2cm or differentiate into a certain number, these young seedlings are taken out under aseptic conditions for rooting culture or re-induction of differentiation.

4. Rooting and culture. When the seedlings in the culture flask grew to about 2cm, the seedlings were taken out under aseptic conditions and cultured in a culture flask equipped with rooting medium. After about half a month, the seedlings can take root.

5. Refining seedlings. When the roots of the seedlings grow to a certain extent and the seedlings are strong, take out the seedlings and wash the culture medium attached to the roots in clean water (note: it must be washed strictly, otherwise the roots will rot). Arrange the washed seedlings, spray them with clean water, and refine the seedlings for 24 hours at a temperature of 15 ℃-25 ℃ and a humidity of 60% Mel 80%.

6. Transplant. After refining the seedlings, the seedlings were transplanted into a domesticated nursery composed of vermiculite, and the seedlings were sprayed with domesticated culture medium and water regularly every day. After about 15 minutes and 20 days, the seedlings could be transplanted to the nursery depending on the growth of the seedlings.