Cultivation of Ganoderma lucidum by Taxus mairei bark

Ganoderma lucidum is a traditional fungal medicine in ancient China, which has good health care and medicinal effect.

Paclitaxel is considered to be a natural anticancer drug discovered in recent 30 years. It has been used to treat a variety of cancer diseases in more than 40 countries. Taxus has become the main tree species in the production of paclitaxel drugs. The bark of Taxus is the organ with the highest taxol content. The cultivation of mycelium and fruiting body of Ganoderma lucidum with yew bark as clinker can achieve better results.

1. Technological process

Taxus bark collection and finishing-- cutting off bundles-soaking bags-sterilization-mycelium culture-ventilation and cooling management-harvest and drying-graded packaging.

2. Cut and finish the bundles

Taxus bark is generally 20 cm long, with a round diameter of 15 cm after each bundle is bandaged, and each bundle is tightly packed inside and outside with fragmentary bark, leaving no space. When bundling, the noodles should be dry, then soak in 1% brown sugar water for more than 6-8 hours, then pour out the water, filter dry, and put it into a 17 × 35 cm bacterial bag for sterilization.

3. Sterilization, inoculation and culture should be strictly in accordance with aseptic operating rules.

Sterilized under 100 ℃ atmospheric pressure for 14-16 hours, if sterilized by high pressure steam, it will be kept for more than 2 hours under 1.5kg/cm2 pressure.

The inoculation room should be cleaned and sterilized before inoculation. The tools and supplies for receiving bacteria, together with the culture bags whose temperature drops below 30 ℃ after sterilization, are all sterilized in the inoculation room, and the operators must receive bacteria carefully and carefully in accordance with the aseptic requirements.

The management of the culture room is mainly in the following three aspects: ventilation, cooling and prevention of miscellaneous bacteria. The effect of reducing pollution can be achieved by properly dealing with ventilation and cooling. The inspection and management of the culture room is related to whether the hyphae can be planted in the bark or the growth after planting. It is also an important factor affecting the yield and quality of Ganoderma lucidum.

4. Zhi Zhi management

When the mature bacterial bag is kept at about 25 ℃, it usually takes 15 days to produce Ganoderma lucidum. The top of the bud grows white and the base is brown. When the stalk reaches a certain length, the cap can be differentiated when the ventilation, temperature and light conditions are available, and the cap develops horizontally. Ganoderma lucidum management is carried out around three aspects: moisture, ventilation and light, especially moisture and ventilation.

5. Ganoderma lucidum fruit body harvest

The symbol of maturity of ① Ganoderma lucidum fruiting body. When the cover is no longer expanded, the color of the cover is the same as the stalk, the edge of the cover is the same as the lid, the cover is thick, and a large number of brown spores are scattered, it can be harvested.

② can be cut or picked from the base of the stalk with fruit tree shears or hands when harvested. The hands of the fresh fruiting body should not be pressed under the lid, do not make the fruiting body collide with each other, cannot be washed with water, cut off the overlong stalk, remove the impurities at the base of the stalk, and dry or dry.

The fungal stalk should also be removed after the fruiting body is removed, otherwise small or deformed fruiting bodies will soon grow from the top of the old stalk.