Biochemical reaction induced by tea cake pathogen in tea leaves
The activities of alanine ammonia lyase, polyphenol oxidase and peroxidase and the contents of protein, proline and phenols in tea leaves infected with tea cake pathogen were studied. The results showed that in diseased leaves, the activity of phenylalanine ammonia lyase decreased, while the activity of peroxidase and polyphenol oxidase increased. The analysis of peroxidase isozymes with benzidine as matrix by polyacrylamide gel electrophoresis showed that there were 4 isozymes in healthy tea leaves and 5 isozymes in diseased leaves. The content of proline in diseased leaves increased significantly, but decreased in the early stage of infection. SDS-PAGE analysis showed that leaves would lose a certain amount of protein when they were infected. Compared with healthy leaves, the total phenolic compounds in diseased leaves increased significantly. According to Agar cup analysis, phenolic compounds showed antifungal activity in both healthy and diseased leaves. The antifungal activity of healthy leaves is higher. HPLC analysis of catechin compounds extracted from healthy leaves and diseased leaves showed that there were qualitative and quantitative differences between the two leaves.
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Introduction of tea plant
The introduction of tea trees is simple and effective. Since the 1950s, the planting area of improved varieties has been expanded. For example, Yunnan big-leaf species have been introduced to Guangdong, Guangxi, Sichuan, Guizhou and other provinces, with an area of more than 1 million mu. Fujian, Zhejiang and other provinces have bred a number of excellent and high-yielding new varieties by using Yunnan big-leaf species introduced and domesticated as raw materials. New progress has been made in the introduction of tea from the south to the north. The northernmost boundary of tea distribution in China has moved from 34 °40 °N to 37 °49 °N, advancing more than 350 km northward. And successively from the former Soviet Union and Japan
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Tea tree pruning requirements
Trim the tea tree to trim the crown of the tree. When light pruning is carried out, the sturdy branches protruding in the middle are 3-5 cm lower than the plane. During the winter pruning time, the low mountain tea garden was carried out around Greater Cold, and the high mountain tea garden was carried out around the Beginning of Spring.
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