A simple and rapid method of producing bacteria in Pleurotus ostreatus production bag
Bag cultivation of Pleurotus ostreatus usually takes 25 to 30 days after inoculation before the mycelium can fill the bag. The longer the time of infection, the greater the probability of infection of miscellaneous bacteria, and the longer the production cycle. In order to shorten the time of collecting bacteria and improve the economic benefit, this paper introduces a simple method of rapid bacteria distribution in Pleurotus ostreatus production bag.
In the center of the two ends of the Pleurotus ostreatus bag, the hole is punched with a sharp rod with a diameter of 2 cm to 3 cm and a length of 30 cm, and the depth of the hole is more than half of that of the bag. Then put an appropriate amount of bacteria into the rod hole, and also put 10% more bacteria than the conventional inoculation amount on the material surface of the bag end, to ensure that the material surface and all around are covered with bacteria. Then seal it with an 8cm × 8cm newspaper and a rubber band. Then the other end is inoculated in the same way, and the bacteria can be infected.
The bacteria should be adjusted according to the temperature. In the high temperature season, the Pleurotus ostreatus production bags are placed in the shape of "mouth" or "well", with 4-5 layers in each row, leaving a certain gap between the row and the row for ventilation and heat dissipation; in the medium and low temperature season, the Pleurotus ostreatus production bags are placed layer by layer, each row has 5-6 layers, and there is also a certain gap between the row and the row for air circulation.
In the germicidal stage, temperature is critical. A few days before the emergence of bacteria, the temperature was controlled at 26-28 ℃ to promote mycelium germination. After the mycelium germinated and ate for 1 day, the temperature should be controlled at 24: 26 ℃. The temperature should not be too low, otherwise it will prolong the sterilization time, but it should not be too high, otherwise the hyphae will be burned out. The management of light, air relative humidity, ventilation, disinfection, bag flipping and so on is carried out in accordance with the routine.
The key point of this method is to drill holes and inoculate and increase the amount of inoculation. the key point of the technology is to master the moisture of the bag material, and it is appropriate to have a moisture content of 65%. The strain has no aging pollution, the hyphae are white and stout, the growth is dense, the vitality is strong, the growth is exuberant, the inoculation room (box) and inoculation tools are strictly disinfected, and the inoculation process is standardized.
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What about the lack of water in the culture material in the later stage of Pleurotus ostreatus production?
The output of the first stubble of Pleurotus ostreatus accounts for about 40% of the total output. Generally, after the second crop of mushrooms is harvested, due to the depletion of water in the culture material, it is rare to produce mushrooms again, the mushroom body is thin, the mushroom meat is loose and dry, and the taste is poor. In order to increase the yield, the following methods can be taken to supplement water and nutrient solution: (1) vertical bag hole irrigation method: the immersion method needs to use a container to hold water, but this method can be used because of the small container or other reasons. Stand the mushroom bag upright on the floor of the mushroom room and pull up the edge of the plastic bag to store water after adding water.
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What are the characteristics of artificially cultivated Gastrodia elata
(1) small investment. Generally speaking, the total expenditure in that year (per square meter) is 27,28 yuan, while the expenditure in the second year is only about 4 yuan, because there is no need to buy hemp seeds and batch strains in the second year. If it is green bark tussah and other hard miscellaneous wood, can be cultivated for 3 years, cork can also be used for more than 2 years. Polyporus umbellatus can also be cultivated with old fungus materials in order to achieve comprehensive utilization and improve economic benefits. (2) the effect is quick. It takes 8 to 10 months indoors and only 1 year to harvest outdoors with white hemp as seed. (3) material
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