What tools and equipment are needed for cultivating Armillaria mellea in Gastrodia elata

Bacteria culture is a highly technical and meticulous work, which must have considerable technical level and necessary equipment and tools. The main equipment and tools required are:

① sterile box: aseptic box, also known as inoculation box, is a small box made of wood and glass, which can be airtight and easy to fumigate with drugs to prevent miscellaneous bacteria infection. The front and rear of the aseptic box is equipped with open and closed glass windows, two holes are opened below, and cloth covers are installed at the opening, and both hands can be extended into the box to operate. The top of the box is equipped with fluorescent lamp or ultraviolet sterilization lamp.

② sterile room: like the sterile box, it should be tightly closed and easy to disinfect. Because of the larger area, the requirements are more stringent. Only when the production quantity is large, the inoculation room should be used.

③ sterilization pot: the sterilization pot is a closed pot, which can increase the steam temperature in the pot and kill miscellaneous bacteria to achieve the purpose of sterilization. According to the scale and economic conditions, high-pressure sterilizer or automatic soil steamer can be used. The earth steamer can be built by brick-wood structure or by brick structure alone.

④ spore collector: it is a device for collecting spores of fungi, including petri dish, perforated bell cover, tripod and so on.

⑤ strain bottle: it is best to use a colored glass bottle, which can not only shade the light but also facilitate the inspection of mycelium growth and the presence of miscellaneous bacteria in the bottle. The capacity of the bottle is about 750ml and the caliber is about 3cm. If it is too large, it is easy for miscellaneous bacteria to invade and it is difficult to keep warm. If it is too small, it is difficult to load and it is inconvenient to take seeds.

⑥ disinfection drugs: formaldehyde (formalin), carbonic acid, potassium permanganate, ethanol (alcohol), bromogeramine (Lysol), mercury and so on.

⑦ medium supplies: potato, Agar (also known as cabbage), glucose, potassium dihydrogen phosphate, magnesium sulfate and so on.

⑧ other items: inoculum needle, tweezers, alcohol lamp, test tube, triangle bottle and ultraviolet lamp, etc. Inoculation needles can be made with stainless steel strips.