Preparation of Pleurotus ostreatus

The production method of Pleurotus ostreatus is basically the same as that of mushroom and straw mushroom. Pure bacteria can be obtained by tissue isolation and spore isolation, and the new area mainly depends on the introduction of mother or original species. The culture medium suitable for the production of Pleurotus ostreatus mother is as follows:

1. Mother medium

1. Maltose yeast Agar medium (MYA): soy peptone (soy peptone) 1g, yeast 2g, maltose 20g, Agar 20g, add water to 1000 ml.

2. Potato glucose yeast Agar medium (PDYA) 300g potato (add 1500 ml of water, boil for 20 minutes, filter juice), 2 g yeast, 1 g peptone, 10 g glucose, 20 g Agar, add water to 1000 ml.

3. Oat flour maltose yeast Agar medium (DMYA): oat powder 80g, maltose 10g, yeast 2g, Agar 20g, add water to 1000 ml.

If no Agar is added in the above three formulations, they can be used as liquid medium. The above culture media need to be routinely prepared, sub-packed, sterilized, inoculated and cultured.

2. Culture medium for original and cultivated species

1, @ # @ 245 straw or naked straw, chopped (2mi 3cm long), soaked, bottled, autoclaved and set aside.

2. Soak wheat, rye, sorghum, corn, millet and other grains, boil thoroughly until there is no white core but the skin does not break, add 2% calcium carbonate, bottled and sterilized under high pressure.

3. 40% of sawdust and 40% of small wood chips and 20% of wheat bran to make culture medium.

The scraps of Pleurotus ostreatus or Flammulina velutipes can also be used as medium for re-sterilization.

III. Vaccination

It can be inoculated with liquid bacteria cultured for 4 days. If solid bacteria are used, the amount of inoculation must be increased (strain / culture material), at least 10%, preferably 15%, 20%.

IV. Training

After inoculation, put the strain bottle or bag in 20Mel 28 ℃ culture room. After the mycelium growth of Pleurotus ostreatus for a few days, the mycelium growth rate will gradually slow down, and the way to accelerate the mycelium growth is to stir. The aseptic wheat grain medium inoculated with liquid bacteria shakes the bottle every 3 seconds and breaks the mycelium, which can stimulate mycelium regeneration and ensure the exuberant growth of mycelium.