Brief introduction of the efficacy of Manni Alkali spermatin in killing Fish parasites

Mannichbasesingredient is a highly effective biological insecticide extracted from the roots and leaves of pure plant eucalyptus and made by scientific processing. People have known that eucalyptus has medicinal value more than 1000 years ago, especially in the rural areas of southwest China, its roots and leaves are usually soaked into solution to directly kill maggots and other pests. In recent years, after in-depth research, relevant departments at home and abroad have refined the preparation, and it is recognized as a biological insecticide with broad spectrum, high efficiency, low toxicity, easy degradation, no residue, no "three causes" and no drug resistance. we have applied it to kill fish ring worms, ciliates and other aspects, and achieved satisfactory results. Now the brief introduction is as follows, for reference.

According to the analysis, the main active components of this product are mannitine (1.8-cineole cineole), 2-pinene (2-pinene), camphene (L-campnere and Cinnamomnncamphora, etc.). Among them, the effect of mannitine is prominent, and the effect of combining aromatic ring side and long chain aliphatic substances is more significant. Its concentration of 0.005 mg / L can kill the third generation of worms and ring worms within 1 hour.

The permeability of Manni alkali semen is very strong, and it is a highly permeable material. It can effectively penetrate the chitin and collagen membrane on the body surface of insects, causing dysfunction and atrophy of the insect body, thus destroying the enzyme activity in the insect body. The experiment shows that it is very effective in killing the population of melons with cysts. In addition, this product contains a kind of peroxide substance, which can produce free radicals that are sensitive to worms, causing worm body deformation, atrophy and death, but harmless to fish, shrimp, livestock, poultry, humans and so on. Professor fick, a famous American toxicologist, has proved that it has a rapid fatality rate to worms by calculating the model C=Coe-KT equation.

I. Test materials and methods

1. The stability of the drug was tested under normal temperature seal for 1, 3, 6 and 18 months. The determination method was determined by rapid thin layer chromatography (TLC). The colorimetric determination was carried out with organic solvent. The results show that the active ingredients are 2%, 5% and 7% respectively, which shows that their properties are stable. It has been preserved for 18 months to meet the product standards.

two。 The practical materials for the pest control test were collected from the extracted Manni alkali concentrate crystals with an effective content of 8%, and the experimental European eel and American eel seedlings (with an average of 7 worms per Gill) were collected from Fuqing and Hefei two eel farms. The experimental containers were filled with 50 kg of water, and the parallel concentrations of 0.05, 0.1, 0.3, 0.4 and 0.5 mg / L were set. They were examined by microscope every two hours and observed continuously for 120 hours. The results showed that the insecticidal rate reached 60% after 24 hours, and the 60-hour fatality rate was more than 90%. Then in the experiment of raising eel with running water in cement pond, the medicine was used twice a day. Once in the morning, the replenishment amount reached 0.4 mg / L after the water was released in the afternoon, and the fatality rate was 95% / 100% after 72 hours. The experiment in many mixed culture ponds with a total of more than 1500 mu showed that the cure rate of ring worms and rotifers was 95% and 100% with the concentration of 0.05 mg / L and 0.1 mg / L.

3. The behavioral response and safety concentration of fish were determined by using eel species as materials, and the behavioral responses to 0.2 mg / L, 0.4 mg / L and control groups were determined by fish avoidance trough. the results showed that the three concentrations had no effect on the behavior of eel species. In addition, we also use the evaluation method of fish safety concentration put forward by American scholar reineye, that is, a cement pond of 120m2 and an average water depth of 1m are used for parallel experiments. After continuous observation for 10 days, compared with the control pool, it showed that it did not have any adverse effect on their eating and swimming.

4. The method of chromosome monosomic recrossing (SCE) in isolated hepatocytes of animals was used to compare this product with the control group. The results showed that no damage and variation of chromosome DNA and the increase of SCE in vivo were found, which confirmed that the product had no effect of inducing "teratogenicity, carcinogenicity and mutagenesis".

II. Preliminary evaluation

In 2006, parasitic diseases of fish ciliates were common in major farming areas of the country, and conventional drugs have caused serious drug resistance of these parasites. The main body of Mannis alkaloids derived four special drugs to kill parasites, such as Japanese eel, European eel, American eel, grass, silver carp, crucian carp, carp and other domestic fish in shrimp and crab mixed culture ponds.