Several methods for quality Identification of Edible Fungi
1 appearance identification
For users without special equipment, they can identify the advantages and disadvantages of bacteria through their senses, that is, the so-called appearance identification. High-quality bacteria should have five characteristics: pure, positive, strong, moist and fragrant.
Pure refers to the high purity of bacteria, no miscellaneous bacterial infection, no plaque, no inhibition line, no "sterilization" and "disruption" phenomenon. It means that the hyphae are normal and have the morphological characteristics of authentic parents. For example, the mycelium is pure white, shiny, growing evenly and neatly, connected into blocks, elastic and so on. Strong means that the mycelium develops sturdily, grows vigorously, has many and dense branches, recovers on the culture medium and eats food quickly. Moistening means that the water content of the strain is moderate, the culture medium is moist, it is close to the wall of the bottle (or bag), and there is no dry shrinkage, loose and liquid accumulation.
2 heat resistance test
The mother seed after rotating tube was cultured at the appropriate temperature for one week, taken out and cultured at 35 degrees Celsius, and then returned to the optimum temperature 24 hours later to observe the recovery of mycelium. It is better to recover quickly and the hyphae lodging and yellowing less.
(3) the tapered bottled fluid medium (such as PDA medium without Agar) was used for line prevention and growth determination. After sterilization, the tested bacteria were inserted into the tested strains after sterilization and cultured at about 25 degrees Celsius for about a week. If bubbles and bacterial membranes occur and have sour taste, it shows that there are miscellaneous bacteria in the bacteria. If there is no above phenomenon, the bacteria will be pure and clean. Then observe the bacteria floating on the liquid surface, if the mycelium grows fast, the hyphae are strong, powerful, exuberant, and the edges are neat and thicker, it shows that the growth potential of the strain is strong; if the mycelium grows slowly, sparse and the mycelium layer is thin, it shows that the growth is weak and should not be used in production.
(4) cultivation experiment identification
If the culture material is packed in a wooden box, if the needle-like hyphae can be seen on the culture material 2 days after sowing, and grow around regularly, and spread to the new culture material 4 days later, while the hyphae on the original bacteria continue to develop around and do not wilt, this is a healthy cultivation species that is fresh and easy to survive.
5 experiment of mushroom emergence
On the well-developed hyphae, cover the large soil particles 3.3cm thick, small soil particles 1.6cm thick, and adjust the water, observe its growth to the soil particles. If the young buds can be seen in the mycelium cover layer at the temperature of 18 Mel 20 degrees Celsius, it is normal to produce mushrooms. If the interval between the first fruiting bodies and the second fruiting bodies is very short, it is a high-yielding species.
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A simple and practical method for Preservation of Edible Fungi
While domesticating the wild domesticated Pleurotus ostreatus strain "1015", we trained them for salt tolerance, that is, the ordinary PDA medium was salinized, prepared into a solution with a concentration of 1.0%-1.5%, sterilized under high pressure for 45-60 minutes, cooled into a slant, inoculated under aseptic conditions, and cultured in an incubator. After the culture is mature, every 10 sticks are packed in a polyethylene bag and stored at room temperature for 9 months. The survival rate is more than 95%.
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New techniques for cultivation of hericium Erinaceus with waste bacteria
Hericium Erinaceus gets its name because it looks like hericium Erinaceus and tastes delicious. It is known as "Shanzhen hericium Erinaceus, seafood bird's nest". In the process of strain production or transfer, sometimes contaminated bacteria will appear due to carelessness in sterilization, disinfection, inoculation, culture and so on. If the contaminated bacteria are abandoned in the wild, it will cause environmental pollution; if you take out the culture medium and wash the bacteria bottle for use, it will be labor-consuming and time-consuming. However, if we use polluted bacteria to cultivate hericium Erinaceus, we can turn waste into treasure and get twice the result with half the effort. The specific methods are as follows: 1. Collect contaminated bacteria and sterilize. Put the contaminated bacteria in time
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