MySheen

Several New methods to reduce the contamination rate of Edible Fungi

Published: 2024-11-22 Author: mysheen
Last Updated: 2024/11/22, 1. The improvement method of bottled fermentation material (1cm) is as follows: the top of the culture medium is about 4cm away from the mouth of the bottle, put into the fermentation material of 1-2cm, and then sterilized. Because the fermentation material itself is sterile, after entering the strain, the hyphae soon cover the upper part of the bottle, and the hyphae are eaten at the same time. The advantage of this method is that the mixed bacteria are not easy to infect the culture medium, and the bacteria age of the strains is the same. The pollution rate can be reduced to less than 3%. Second, the improved method of wheat grain germination is to let the wheat grains germinate and then bottle and sterilize. The main storage substances of wheat grains are starch, fat and protein.

1. Bottled fermentation material (1cm)

The improved method is: 1- 2 cm of fermentation material is filled in the upper part of the culture medium about 4 cm away from the bottle mouth, and then sterilized. Because the fermentation material itself is sterile, after the inoculation of the strain, the mycelium quickly covered the upper part of the whole bottle, and the mycelium ate the material at the same time. The advantages of this method are that miscellaneous bacteria are not easy to infect the culture medium, and the bacterial age of the strains is consistent. The pollution rate can be reduced to less than 3%.

2. Wheat germination method

The method of improvement is to germinate the wheat grains and then bottle them for sterilization. Starch, fat and protein are the main storage substances of wheat grain. When the seed imbibes and the physiological activity of cells is reactivated, the activity of various enzymes is enhanced. These substances are decomposed into maltose, sucrose and amino acids with simple structure during germination, which are easy to be utilized by hyphae. The specific method is as follows: the wheat grains used as the strains are allowed to germinate at one time, the length of the buds is 1-2 times of the length of the wheat grains, then the wheat grains are fully dried and stored, the wheat grains are soaked for 12 hours one day before the strains are used, and the wheat grains are directly bottled and sterilized. The method has the advantages of omitting the process of boiling wheat grains, saving manpower and financial resources, rapid bacterial growth, less miscellaneous bacteria, and pollution rate of only 2%-3%.

III. Use of liquid strains

The improvement method is: using liquid strain. Liquid strain overcomes the shortcomings of solid strain, liquid strain can be directly cultivated as a cultivated species. The use of liquid strains not only has a low probability of infection by miscellaneous bacteria, the pollution rate can be reduced to about 2%, but also from a long-term perspective, because it is produced without plastic bags, there is no pollution to the environment, and the production cost of strains is reduced.

IV. Inoculate bacteria from the lower part of the original seed bottle

The method of improvement is to inoculate from the bottom of the stock bottle. We know that the strain in the lower part of the original seed bottle has short age, vigorous vitality, and less miscellaneous bacteria. After inoculation, the mycelium eats quickly and the pollution rate is low, so the pollution rate can be reduced to about 3%. In order to further reduce the contamination rate of cultivated seeds, it is suggested to discard 1/5 of the upper part of the normal original seed bottle mouth to prevent the contamination of cultivated seeds by miscellaneous bacteria invisible to the naked eye.

V. In high temperature season, inoculation time is advanced or delayed

The improvement method is: inoculating bacteria in the lower temperature period of July and August, such as morning or evening. Of course, if the indoor inoculation effect is better, the pollution rate can be reduced to less than 5%.

 
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