Several New methods to reduce the contamination rate of Edible Fungi
1. Bottled fermentation material (1cm)
The improved method is: 1- 2 cm of fermentation material is filled in the upper part of the culture medium about 4 cm away from the bottle mouth, and then sterilized. Because the fermentation material itself is sterile, after the inoculation of the strain, the mycelium quickly covered the upper part of the whole bottle, and the mycelium ate the material at the same time. The advantages of this method are that miscellaneous bacteria are not easy to infect the culture medium, and the bacterial age of the strains is consistent. The pollution rate can be reduced to less than 3%.
2. Wheat germination method
The method of improvement is to germinate the wheat grains and then bottle them for sterilization. Starch, fat and protein are the main storage substances of wheat grain. When the seed imbibes and the physiological activity of cells is reactivated, the activity of various enzymes is enhanced. These substances are decomposed into maltose, sucrose and amino acids with simple structure during germination, which are easy to be utilized by hyphae. The specific method is as follows: the wheat grains used as the strains are allowed to germinate at one time, the length of the buds is 1-2 times of the length of the wheat grains, then the wheat grains are fully dried and stored, the wheat grains are soaked for 12 hours one day before the strains are used, and the wheat grains are directly bottled and sterilized. The method has the advantages of omitting the process of boiling wheat grains, saving manpower and financial resources, rapid bacterial growth, less miscellaneous bacteria, and pollution rate of only 2%-3%.
III. Use of liquid strains
The improvement method is: using liquid strain. Liquid strain overcomes the shortcomings of solid strain, liquid strain can be directly cultivated as a cultivated species. The use of liquid strains not only has a low probability of infection by miscellaneous bacteria, the pollution rate can be reduced to about 2%, but also from a long-term perspective, because it is produced without plastic bags, there is no pollution to the environment, and the production cost of strains is reduced.
IV. Inoculate bacteria from the lower part of the original seed bottle
The method of improvement is to inoculate from the bottom of the stock bottle. We know that the strain in the lower part of the original seed bottle has short age, vigorous vitality, and less miscellaneous bacteria. After inoculation, the mycelium eats quickly and the pollution rate is low, so the pollution rate can be reduced to about 3%. In order to further reduce the contamination rate of cultivated seeds, it is suggested to discard 1/5 of the upper part of the normal original seed bottle mouth to prevent the contamination of cultivated seeds by miscellaneous bacteria invisible to the naked eye.
V. In high temperature season, inoculation time is advanced or delayed
The improvement method is: inoculating bacteria in the lower temperature period of July and August, such as morning or evening. Of course, if the indoor inoculation effect is better, the pollution rate can be reduced to less than 5%.
- Prev
Comparative experiment on liquid strain substitute cultivation of six strains of Lentinus edodes
In recent years, the production of Lentinus edodes in China has developed rapidly, especially the substitute cultivation of Lentinus edodes. The traditional solid seed production technology of Lentinus edodes can be extended to the production of cultivation bags after the three-stage cultivation of mother seed, original seed and cultivated species. The development of Lentinus edodes is greatly restricted by its great labor intensity, complicated technology, high contamination rate of miscellaneous bacteria, long growth cycle and so on. In this experiment, six liquid strains of Lentinus edodes were cultured and cultivated, in order to provide a theoretical basis for the popularization and application of liquid strains of Lentinus edodes. 1 Materials and methods 1
- Next
A simple and practical method for Preservation of Edible Fungi
While domesticating the wild domesticated Pleurotus ostreatus strain "1015", we trained them for salt tolerance, that is, the ordinary PDA medium was salinized, prepared into a solution with a concentration of 1.0%-1.5%, sterilized under high pressure for 45-60 minutes, cooled into a slant, inoculated under aseptic conditions, and cultured in an incubator. After the culture is mature, every 10 sticks are packed in a polyethylene bag and stored at room temperature for 9 months. The survival rate is more than 95%.
Related
- Fuxing push coffee new agricultural production and marketing class: lack of small-scale processing plants
- Jujube rice field leisure farm deep ploughing Yilan for five years to create a space for organic food and play
- Nongyu Farm-A trial of organic papaya for brave women with advanced technology
- Four points for attention in the prevention and control of diseases and insect pests of edible fungi
- How to add nutrient solution to Edible Fungi
- Is there any good way to control edible fungus mites?
- Open Inoculation Technology of Edible Fungi
- Is there any clever way to use fertilizer for edible fungus in winter?
- What agents are used to kill the pathogens of edible fungi in the mushroom shed?
- Rapid drying of Edible Fungi