How to prevent degradation of edible fungi strains
The degradation of bacteria will lead to the slow growth of mycelium, the weakening of resistance to environment and miscellaneous bacteria, the advance or postponement of fruiting body formation, the unobvious tide of mushroom, and so on. There are many reasons for strain degradation, among which impurity and gene mutation are the main reasons. In addition, high temperature and mixed cultivation of different strains will cause strain degradation.
The measures to prevent the degradation of bacteria are as follows: to ensure the pure culture of bacteria, to be careful not to use the strains contaminated by miscellaneous bacteria, not to connect them closely, to strictly control the passage times of bacteria, to reduce mechanical damage, and to ensure the vitality of bacteria; to preserve bacteria at low temperature, such as mushrooms and shiitake mushrooms, to preserve the vitality of mycelium at 4 ℃, and to preserve the vitality of mycelium at 16 ℃ for high temperature strains such as straw mushroom. Avoid multiple passages in a single culture medium; strains should not be used for too long, over-aged strains will appear aging, and aging and degradation are organically linked, and strains with weak vitality are easy to degenerate; bacteria should be rejuvenated regularly under the conditions of suitable temperature, suitable pH, sufficient oxygen, no miscellaneous bacteria and so on. Spore isolation is carried out every year, excellent strains are found by sexual reproduction, and tissue isolation is used to consolidate the genetic characteristics of excellent strains.
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Preservation of edible mushroom strains
Usually take the following two methods of preservation: slope low temperature preservation method. This is the simplest preservation method, that is, the vigorous and pollution-free slant test tube is put in the refrigerator and preserved at a low temperature of 4 ℃ ~ 6 ℃, and then transferred to the fresh slant medium every 2 to 3 months, and then preserved after culture. The other is liquid paraffin preservation. This method is to inject sterilized liquid paraffin in the aseptic room in the inclined test tube to be preserved, and it is appropriate to inject wax 1 cm higher than the inclined surface. Then plug it with a cork or rubber.
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Comparative experiment on liquid strain substitute cultivation of six strains of Lentinus edodes
In recent years, the production of Lentinus edodes in China has developed rapidly, especially the substitute cultivation of Lentinus edodes. The traditional solid seed production technology of Lentinus edodes can be extended to the production of cultivation bags after the three-stage cultivation of mother seed, original seed and cultivated species. The development of Lentinus edodes is greatly restricted by its great labor intensity, complicated technology, high contamination rate of miscellaneous bacteria, long growth cycle and so on. In this experiment, six liquid strains of Lentinus edodes were cultured and cultivated, in order to provide a theoretical basis for the popularization and application of liquid strains of Lentinus edodes. 1 Materials and methods 1
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