Preservation of edible mushroom strains
Two methods of preservation are usually used: Cryopreservation. This is the simplest preservation method, that is, the vigorous and pollution-free slant test tube is placed in the refrigerator, stored at a low temperature of 4℃~6℃, and then transferred to a fresh slant culture medium every 2~3 months. After cultivation, it will continue to be preserved. The other is liquid paraffin preservation. This method is to preserve the slant in the test tube, in the sterile room injection of sterilized liquid paraffin, wax injection to 1 cm above the slant is appropriate. Then plug with a wooden stopper or rubber stopper, sealed with wax, upright into the indoor dry place, has been preserved for more than one year.
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Preservation of Edible Fungi
1. Distilled water method. It is suitable for Pleurotus ostreatus, Flammulina velutipes and Lentinus edodes. 1. Preserve Pleurotus ostreatus and Flammulina velutipes. After 80% of the bacteria to be preserved in the test tube were transferred into the inoculation box for sterilization, the medicinal syringe was injected with steamed water from the test tube plug to submerge the culture medium to 2 cm at the mouth of the tube. The mouth of the tube is sealed with mineral wax and stored in a cool place at room temperature for 1 to 1.5 years. two。 Preserve Lentinus edodes and fungus strains. A 150x15mm finger tube will be used to culture medium well's solid or
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How to prevent degradation of edible fungi strains
Strain degeneration will lead to slow growth of mycelium, weak resistance to environment, miscellaneous bacteria, etc., fruit body formation period ahead or behind, fruiting tide is not obvious and so on. There are many reasons for strain degeneration, among which impure strain and gene mutation are the main reasons. In addition, high temperature and mixed cultivation of different strains will cause strain degeneration. Measures to prevent strain degeneration include: ensuring pure culture of strains, paying attention not to use strains contaminated by miscellaneous bacteria, not to connect and culture in close proximity; strictly controlling the passage times of strains, reducing mechanical damage,
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