Brief introduction of production and Application Technology of Lentinus edodes capsule

At the end of the last century, glue patrol bacteria were invented in Japan and widely used in Lentinus edodes. This technology was introduced to China in 1999. After years of research, Qingyuan County Edible Mushroom Research Center has overcome the technical problem of the application of capsule strains in Lentinus edodes, and realized domestic production. Because the capsule strain has the advantages of labor-saving, labor-saving and high survival rate, it is very suitable for large-scale production. In the past two years, the amount of application has doubled, and tens of millions of bags have been popularized and used. it is estimated that this technology will be widely used in China in a few years' time. In order to make people in the industry better understand this technology, the author introduces the main characteristics, production technology and usage of capsule bacteria as follows.

1 the main characteristics of capsule bacteria

Conventional bacteria are bagged or bottled, which are inconvenient to use, have long exposure time in the air, have a high chance of infection with miscellaneous bacteria, and the survival rate of inoculation is relatively low. On the other hand, the size of the inoculation hole of conventional bacteria is different, and the amount of seed is not easy to control, the gap of the inoculation mouth is often large. In the process of handling and operation of the stick, artificial extrusion causes air to go in and out of the gap and carry miscellaneous bacterial spores into the rod, causing infection.

Lentinus edodes capsule bacteria, just like capsules, are pressed on the plastic honeycomb board one by one, and each strain is tapered, and the tail end is adhered to a breathable foam cover, which is scientific in design, standard and convenient for access; the inoculation operation process has short contact time with air and less chance of pollution; after inoculation, the foam cover is sealed and breathable, which can not only prevent the infection of miscellaneous bacteria and diseases and insect pests, but also maintain the moisture of the bacteria and promote the good development of mycelium. Therefore, the yield of finished products can be greatly improved compared with traditional strains. The inoculation of capsule bacteria is fast, the work efficiency is 1-2 times higher than that of conventional bacteria, and the raw material consumption of producing bacteria is reduced by more than 80%. Because the capsule strain was crushed and pressed on the plastic honeycomb board, the species did not change, and the quality and yield of the mushroom were not different from those of the traditional strain.

2 capsule strain and production technology

2.1 the formula of special culture material is different from that of conventional bacteria, one is that fine material is required, which is too thick and easy to cause damage to the hole plate, and the other is that it not only has better water retention effect, but also has better permeability.

2.2 bagging sterilization is the same as routine sterilization.

2.3 inoculation is the same as routine.

2.4 Culture of bacteria should be carried out under completely dark conditions to the extent that the bacteria are mature and can not afford the skin.

2.5 the link of aseptic crushing and aseptic packing must be carried out in a sterile room. If miscellaneous spores are mixed into the culture material, the spores will germinate and exceed the hyphae of Lentinus edodes under certain conditions, resulting in inoculation mouth infection. The filler is required to be compact and moderate, and it is easy to break the hole plate if it is too compact. too loose will cause the particles to be not full and affect the quality.

2.6 seed pressing and capping of the acupoint plate are also carried out in the aseptic room, which requires skilled operation and reduces the exposure time when sealing.

2.7 after crushing and molding, the culture package breathes vigorously within 3-5 days, and it is easy to burn bacteria. Therefore, the culture of glue stroll bacteria should be kept relatively low temperature, ventilated, and the stack height should not exceed 10 pieces.

3 key points for attention in the use of capsule bacteria

3.1 the bacteria stick should be bagged tightly to facilitate the colonization of capsule bacteria.

3.2 the inoculation process requires the same strict operation as conventional bacteria. Before inoculation, the operator's hands and instruments should be wiped and disinfected with 75% medicinal alcohol or inoculation spirit, and the alcohol cotton sterilized punching rod should pass through the alcohol lamp flame to avoid alcohol droplets remaining on the rod. The strain shall not be disinfected by aerosol at the same time with the stick to prevent the mycelium from being killed. The foam cover surface of the capsule strain can be smeared with alcohol, but do not allow alcohol to flow into the strain.

3.3 punch a hole and take one capsule after another, and the exposure time of the bacteria in the space is as short as possible. The punch stick can't be put anywhere without leaving it in hand.

3.4 in order to facilitate seed collection, you can first divide the capsule bacteria into small pieces from the reverse side with an one-sided knife. When taking seeds, the right index finger gently press the breathable cover of the bacteria, and the left index finger enters the upper support from the bottom, then gently hold the lid with the right thumb and index finger to remove the bacteria, quickly insert it into the drilled hole, and gently press the lid to seal it with the surface of the bag. Be careful not to touch the bacteria under the lid with your hands.

3.5. because of the good sealing of glue bacteria, when the bacteria are above 4cm, the inoculation mouth is easy to lack oxygen, so it should be punctured in time to increase oxygen to facilitate the bacteria.

3.6 the sterilized Lentinus edodes stick must be cold thoroughly, otherwise it will be hot inside and cool outside, and the moisture will be condensed at the mouth of the inoculation, which will kill the strain.

4 preservation and transportation of capsule bacteria

4.4.The capsule bacteria should be stored in a clean and dark environment, the storage period is about 12 days at 18 ℃, and the capsule bacteria stored in cold storage at low temperature (4 ℃) can be stored for 30 days. The bacteria should be tightly wrapped in plastic bags for cold storage in cold storage. when in use, it must be taken out and activated at room temperature one day in advance before it can be used for inoculation. The capsule bacteria are easy to be dehydrated and should not be preserved for a long time.

4.2 the transport of capsule bacteria is overbreathed before the strain is permeated, so it is not suitable for transportation, and it can not be transported until at least 4 days later. Transport needs to be air-conditioned or refrigerated, ordinary freight can be carried out in winter, and the time on the way is no more than one week.