Production of plastic bag cultivation of Lentinus edodes
The strain production method of 17cm × 33cm polypropylene plastic bag has been introduced in the production of block cultivation. In recent years, the production of substitute Lentinus edodes has developed rapidly in northern China. Due to different climatic conditions and other factors, large bags are mostly used in northern areas. Large bag cultivation also uses plastic bags as containers for strain production, so its operation precautions and technical points are the same, but it is different in some specific operations.
Large bag cultivation using low-pressure high-density polyethylene plastic bag, 0.5 mm thick, 22 cm wide, 55 cm 58 cm long, each bag of dry material 1.5 kg. The formula of culture material: sawdust 80%, wheat bran 20%, gypsum 1.5%, lime 0.2% 0.3%, material / water ratio 1 / 0. 9. It is advisable to hold the material with water marks in the fingers but not dripping. Material bag sterilization is the key technical link of large bag cultivation. Sterilization operation should adhere to the principles of head attack, tail protection and intermediate control, ensuring that the temperature is raised to 100 ℃ in 6 hours, and at the beginning of vigorous fire, pay attention to adding hot water, preventing air leakage, not cooling, keeping 100 ℃ for 18 ℃ for 20 hours, finally burning for 4 hours, and then stuffy for 1 hour. When the temperature drops to 70 ℃, slowly open the door and let out the pot. The sterilization time is positively correlated with the volume of the sterilizer and the thickness of the bacteria stick. With the increase of the pot volume and the thickening of the bacteria stick, the sterilization time should be increased. The sterilization time should be kept at about 100 ℃ for 24 hours, up to 40 hours. Inoculation uses three sides 9 acupoints (3 × 3) inoculation, vaccination should be strict aseptic operation, to prevent miscellaneous bacteria from invading the vaccination point. The bacteria were stacked in "well shape" with a distance of 10 cm and a height of 6-7 layers. Turn the pile for the first time 10-15 days after inoculation, and check the mycelium growth and miscellaneous bacteria infection at the same time, and turn the pile again on the 10th day after inoculation. In the first week, the room temperature was controlled at 25 ℃, and then the bag temperature was controlled at 20 ℃, the bag temperature was 24 ℃, the bag temperature was strictly prevented from exceeding 28 ℃, the air relative humidity was controlled at 60% 65%, Rain Water often released quicklime to absorb moisture, and closed the doors and windows. When the diameter of the inoculation hole reaches 8cm to 10cm, it can increase the ventilation rate and loosen the branches of the bacteria to help ventilate and promote the growth of mycelium.
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Prevention of miscellaneous bacteria infection in the cultivation of Pleurotus ostreatus in North China
As the number of Pleurotus ostreatus production in the north increases year by year, the disease is serious, resulting in a decrease in production in light cases and a failure in heavy ones. In fact, the disease of edible fungi is not an incurable disease. As long as we grasp the law of its occurrence and development and find out its effective prevention and control methods, the problem can be solved. After years of cultivation practice, the author has found out the law of miscellaneous bacteria infection, and now introduces its causes and preventive methods as follows: 1 it is too late to start inoculation until April, because the weather has turned warm and easy to infect miscellaneous bacteria. February-March
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Strain treatment and Culture of Lentinus edodes
The original and cultivated seeds of Xiangru should be stored in a cool and clean storeroom or in a sterilized refrigerator, and vaccination should be carried out in a sterilized inoculation box or inoculation room. When cultivating pest-free cultivation, the culture site should be carried out in a special sterilized shed, and the strain field should be isolated from the production field. The proportion of cultivated seed culture materials is 98% of cottonseed hull, 8% of wheat bran, 1% of lime, 1% of gypsum, 1% of phosphate fertilizer and appropriate amount of water, and 110-130 jin of auxiliary materials are added to mix evenly. The prepared culture material was packed into a strain bag and sterilized under atmospheric pressure at a temperature of 100℃.
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