Key points of management of mycelium growth period of Lentinus edodes

Key points of management of mycelium growth period of Lentinus edodes

The suitable conditions for mycelium growth of Lentinus edodes are temperature 22 ℃ ~ 26 ℃, air humidity 60% ~ 65%, good ventilation, dark or weak light.

1-6 days after inoculation, it was the period of mycelium germination and colonization. Because the mycelium grew weakly, the bag temperature was 1 ℃ ~ 3 ℃ lower than the room temperature. In order to provide the suitable temperature for the mycelium, the room temperature should be adjusted to 28 ℃ ~ 29 ℃. The room temperature, stack temperature and bag temperature should be measured every day. It is not appropriate to move the bacterial bag at this time to prevent miscellaneous bacterial infection. No ventilation or a small amount of ventilation within 3 days.

7-10 days after inoculation, the hyphae spread into the material, the bag temperature increased gradually, and the room temperature was controlled at 25 ℃ ~ 27 ℃. Start to turn the pile after 7 days, and then turn it once every 7 to 10 days. When turning the pile, the mycelium growth and miscellaneous bacteria infection were checked bag by bag. If a missing inoculation bag or inoculation hole is found, it should be concentrated in other places, the mildew point should be removed, and the affected area should be smeared with 5% 10% lime water. The culture room should be ventilated once or twice a day for about 30 minutes each time.

11-20 days after inoculation, the hyphae entered the vigorous growth period, various metabolic activities accelerated, and the bag temperature was 1 ℃ ~ 5 ℃ higher than the room temperature. Special attention should be paid to cooling and ventilation. The room temperature was controlled at 20 ℃ ~ 24 ℃ and ventilated for 1 to 2 hours a day.

20 days after inoculation, oxygen in the bag could not meet the requirements of mycelium growth due to a large number of mycelium growth, and hypoxia became a limiting factor for mycelium growth. The adhesive tape on the inoculation hole should be removed in time, or the oxygen should be increased by piercing holes. The time of the first piercing occurred when the diameter of the mycelium circle was 8 cm to 10 cm. Use sterilized toothpicks to pierce 6-10 holes in front of the mycelium circle, the hole depth can not touch the material layer. When the hyphae between the inoculation points are connected, the sterilized sweater needle is used for the second puncture to increase oxygen. When the mycelium is full, use sterilized chopsticks to pierce holes around the bag. The number and depth of the holes depend on the temperature and mycelium growth. When the temperature is high, more piercing holes, deep piercing holes, on the contrary less piercing holes, shallow piercing holes. After uncovering the tape or piercing holes, the growth of mycelium is accelerated, and the temperature of the bag will increase suddenly. At this time, it is necessary to increase ventilation and evacuate the bacterial bag to prevent burning. After 30-50 days, the hyphae are full of bacterial bags, which can be used for color conversion and physiological maturation management.

The color change of mycelium is a special physiological process of mycelium development of Lentinus edodes, and it is also a sign of mycelium development and maturity. The suitable conditions for the color conversion of the bacterial tube: the temperature is 18 ℃ ~ 24 ℃, the air humidity of the microclimate around the bacterial tube is 80%-85%, there is a certain scattered light and good ventilation conditions. When the mycelium is full of bags, the sunshade should be removed to increase indoor lighting. The room temperature should be strictly controlled at 20 ℃ ~ 24 ℃, the temperature difference should not be too large, and the bacterial bag should be moved less. If cultivated in a large bag, the color conversion and physiological maturation time of the fungus tube is 25: 30 days; if cultivated in a small bag, the color conversion time is 10: 15 days. In this process, the hyphae gradually lodged and secreted brown pigment, which changed the tube from white to brownish red. If the condition of changing color is suitable, the color of the tube is uniform, the thickness of the film is the same, and the mushroom is neat, the yield is high and the quality is good. If the color is too light or uneven, the quality of mushroom is poor and there are many abnormal mushrooms. If the color change is too heavy and the bacterial membrane is too thick, the mushroom will grow slowly and the yield will be low.