Rapid bud induction of Lentinus edodes

The method of water injection or bubble bag budding in bag cultivation of Lentinus edodes will make the water content in the stick too high and affect the yield, quality and benefit. In this paper, a method of rapid bud induction is introduced.

1. Four iron nails 8 cm ~ 10 cm long were used to pierce the hole, which were nailed on a board with a thickness of 1.5 cm, a length of 45 cm and a width suitable for hand-holding. The "nail row" was used to pierce 8 eyes on the bacterial stick.

two。 Soak the pierced stick into a soaking pool or vat to prevent the stick from floating. After that, soak the bacteria stick with 0.3% urea solution.

The sticks of Pleurotus ostreatus were soaked in water for 1.5 hours ~ 2 hours, and the sticks of Pleurotus ostreatus were soaked in bags for 2 hours ~ 3 hours each time. The water content around the piercing hole is 65%-70%, and the water content of other parts is 45%-60%. Generally, the weight of soaking water is determined by weighing method. The weight after the first immersion is 100%-110% of the weight of bacterial stick cultivation, and the spring planting bag is 90%-105% of the weight of cultivation. After that, the amount of water immersed in each tide will be reduced by about 15%.

3. When the temperature difference is 8 ℃ ~ 20 ℃ in sunny day, the low temperature below 12 ℃ must appear at night, and the medium temperature strain needs low temperature stimulation of 14 ℃ ~ 16 ℃. A hole is pierced at one end of the bacterial stick soaked in water, and the hole is downward, discharged on the sunny, leeward, non-stagnant ground, covered with plastic film, and a thermometer is placed on top of the bacterial stick in the film. Sunlight increased the temperature of the film to 28 ℃ ~ 32 ℃, kept it for 2 hours ~ 4 hours, lifted the film for ventilation, and then stimulated with 12 ℃ natural low temperature at night for 4 hours ~ 6 hours. If the temperature is not enough, it can be carried out 2 ~ 3 times in a row.

4. In the management of mushroom production, the stick stimulated by piercing, soaking in water and temperature difference can be placed in place and tightly covered with plastic film, and a large number of mushroom buds can be produced in 2 ~ 3 days under the condition of 18 ℃ ~ 24 ℃. The rods with more mushroom buds were selected from the evening of the second day or the morning of the third day and put into the bottom layer of the mushroom shed. After that, it was selected every 12 hours to 24 hours and put into the mushroom shed from the bottom up, and it was completed in 2 ~ 3 days.

5. Note that the water content of the bacterial stick used by ① to promote bud by the above method should be 35%-55%. If the water content is less than 35%, a small amount of water should be replenished. If the water content is higher than 55%, the immersion time should be shortened or not. If the ② exceeds 35 ℃, the temperature should be cooled immediately.