Cultivation of Lentinus edodes by artificial inoculation

If it is suitable for drying, Lentinus edodes should be inoculated in time. The inoculation time is generally more than 5 ℃, less than 20 ℃, and the monthly average temperature is about 10 ℃. In the south of the Yangtze River in China, Lentinus edodes was inoculated from late February to early April, and the most suitable period was from February to mid-March. There are sawdust bacteria, branch bacteria and wedge-shaped bacteria, and sawdust bacteria are used in most domestic wood cultivation. The high quality pure strain of Lentinus edodes, the mycelium is white and dense, has the unique fragrance of Lentinus edodes, the aerial mycelium has the ability to climb the wall, and the mycelium in the culture medium has no miscellaneous color and obvious inhibition line. For some fast-growing strains, white primordia appear in the bottle before the hyphae reach the bottom of the bottle. Such strains are generally fast-growing and high-product lines. Where the bacteria age is too long, the hyphae turn yellow, the culture medium shrinks and becomes small or dark brown, and the bacteria that fall off with the bottle wall should not be used.

Generally speaking, the inoculum quantity per cubic meter section of water requires 6 strains and 8 bottles of inoculation tools, such as electric drill, hole punch, inoculation axe, hand drill, belt punching and so on. The vaccination method can be divided into three steps:

① inoculation point: the diameter of the inoculation point is 1.2cm, the depth is 1.2cm-1.5cm, and the vertical distance between the acupoints is 15Mel 20cm, and the horizontal distance is 8Mel 10cm. Located at both ends of the cross section and the injured place, it can be more dense to resist the invasion of miscellaneous bacteria, and the holes between the two adjacent rows should be arranged in the shape of plum blossoms in order to produce mushrooms evenly. In order to improve the utilization rate of mushroom wood, it must be planted properly according to the thickness and material of the section wood.

② inoculation: the bacteria should be connected as soon as possible after burrowing to prevent the wall of the cave from drying and miscellaneous bacteria from invading. When inoculating, small pieces of wood should be connected first, then medium and large sections of wood should be connected. If sawdust bacteria, should try to keep block, gently fill into the inoculation hole, to 80% full; if cork bacteria, put into the inoculation hole should be plugged tightly, so that the outer end of the cork and bark into a flat, will not fall off. The strains of Lentinus edodes should be used whenever they are dug. The strains dug up had better be used up on the same day and should not be placed overnight.

③ lid: using sawdust bacteria, after inoculation, cover the bark that has been beaten in advance (1.5mm larger than the inoculation hole) and level it tightly. The seed cover and mushroom wood must be the same tree species, with a thickness of 4mm and 5mm, so as not to fall off as expected by heating. do not cover the bark cover, you can also use a wax seal instead. For cork bacteria, there is no need to cover and wax seal.

The sawdust strain is easy to make, and the survival rate after inoculation can reach more than 90%, while the cork strain is easy to dry and infect miscellaneous bacteria because it is partially exposed after inoculation.