Sterilization and inoculation of Lentinus edodes

Lentinus edodes culture medium mostly uses the method of atmospheric pressure and high temperature sterilization to achieve the purpose of killing pests. (1) bag material sterilization: put the bag in the human sterilization stove and sterilize under normal pressure. Before putting the material bag into the stove, add enough water to the steamer first. When the material bag is loaded into the stove, it should not be installed in the shape of "product". It should be discharged overlapping from top to bottom, leaving a gap between the row and the discharge to make the air flow up and down smoothly. After the bag entered the warehouse, the fire immediately burned, so that the temperature quickly rose to 100 ℃ within 6 hours and lasted for 24 hours. To achieve "attack, tail protection, control in the middle." After meeting the sterilization requirements, open a crack in the warehouse door and let the hot air out slowly. When the temperature in the warehouse drops below 60 ℃, you can unload the bag while it is hot. (2) sterilization in the sterilization room: the fumigation method is to put the tools used out of the pot indoors 2 days before the use of the inoculation room, seal the doors and windows so that there is no air leakage, the ground can splash a small amount of water, and then ignite 1-1.5 kg of sulfur, the sulfur gas produces carbon dioxide after seeing water, and fumigation for more than 20 hours, which can not only sterilize, but also kill insects, mites and rats. After the material bag enters the inoculation room, when the temperature of the bag drops below 30 ℃, put all the tools and tables and stools for inoculation into the room, fumigate and disinfect with formaldehyde for the second time, the dosage of each room is 1-1.5kg, heat and evaporate in a small pot or basin. Make it all gasified, 8-10 hours later, can achieve the purpose of disinfection. The inoculation method generally uses branch bacteria: two people and one group are generally used during inoculation; one person wipes the bacteria bag in a straight line with alcohol cotton, and the other person holds the mouth of the strain bottle in his left hand, so that the tip is vertically inserted into the bag, and the full end is left outside for 3m, which is not allowed to be inserted askew, so as not to infect miscellaneous bacteria. Each bag of "product" is connected with 3 lines, each line is equidistant with 3 holes, and a bottle of bacteria is connected with 10 bags. The inoculated bacterial bags are stacked in the shape of "well" with a height of 5 Mel and 6 layers or placed neatly on the culture rack. Then each room with 500 grams of formaldehyde spilled in the space, closed doors and windows, into the germ period management.