Necessary conditions for the growth and development of Lentinus edodes

1. Nutritious Lentinus edodes is a kind of higher wood rot fungus. The carbon source for its growth comes from lignin, cellulose, nitrogen source and inorganic salt, and is taken from wood bark or wheat bran (rice bran) added to sawdust medium. After Lentinus edodes was inoculated into Lentinus edodes, the culture material was decomposed into small molecular substances which could be absorbed and utilized by the mycelium of Lentinus edodes, and the mycelium biomass increased with the increase of culture time. When the culture material was fully degraded, the mycelium of Lentinus edodes accumulated a lot of nutrients, which laid the material foundation for the occurrence of fruiting body. Under suitable conditions, Lentinus edodes changed from vegetative growth to reproductive growth, the fruiting body began to grow and develop, and a large number of nutrients accumulated in the mycelium of Lentinus edodes were transferred to the fruiting body of Lentinus edodes.

2. Temperature Lentinus edodes mycelium growth temperature is 5: 32 ℃, robust growth temperature is 18: 22 ℃, at this time the mycelium growth rate is slightly slower, but the mycelium is stout and dense; the rapid growth temperature is 24-27 ℃, but the mycelium is fine and sparse; below 0 ℃ and above 32 ℃, the mycelium grows poorly and stops growing at 35 ℃. The mycelium of Lentinus edodes has strong cold tolerance and can survive under 5 ℃ for 8 weeks. If the mycelium of Lentinus edodes lurks and spreads in the section of wood, due to the protection of bark, the short-term high temperature and severe cold will not kill the hyphae or freeze to death, but it has a great influence on the cultivation of sawdust sticks. Lentinus edodes is a kind of fungi with low temperature and variable temperature. When the mycelium reaches physiological maturity, it is stimulated by seasonal change or man-made temperature difference, and the mycelium kink into primordia. The differentiation temperature of Lentinus edodes primordium was 8-21 ℃, and the optimum differentiation temperature was 10-12 ℃. The required temperature difference was determined by Lentinus edodes strains. The primordium differentiation temperature and development temperature of Lentinus edodes strains were 5: 15 ℃ and 10: 15 ℃, 8: 20 ℃ and 15: 20 ℃, respectively, and those of high-temperature strains were 15: 25 ℃ and 20: 25 ℃. After the formation of the primordium of Lentinus edodes, the growth and development of fruiting body does not require high temperature difference, but the appropriate temperature difference can promote the formation of high quality Lentinus edodes. In general, high temperature, rapid development of fruiting body, more thin-skinned mushrooms, poor quality; at low temperature, the development of fruiting body is slow, high quality, thick and fragrant. In production practice, the temperature type of cultivated Lentinus edodes strains should be selected according to the variation range of local air temperature.

3. Too much water content in the humidity culture medium will block the pores between sawdust, resulting in hypoxia leading to hyphae "drowning", while too little water content will make the hyphae unable to absorb enough water for growth and "die of thirst". In the cultivation of Duanmu, the appropriate water content of Duan Mu is 32%, 42%, 35%, 35% and 40%. The optimum water content in sawdust medium is 55% and 60%. The relative humidity of the air should be kept at about 70% in the germicidal stage, and too high humidity is conducive to the growth of miscellaneous bacteria. Humidity is very important to the differentiation and development of fruiting body of Lentinus edodes. In the process of mushroom production management, the relaxation of Lentinus edodes is mainly controlled by adjusting temperature and humidity. The water content of bacterial rods needed for fruiting body differentiation is generally 60%-65%, and the air relative humidity is generally 85%-90%. After the formation of the primordium of the fruiting body, the relative humidity of the small environmental space around the sound development of the fruiting body is 80%-85%.

4. Lentinus edodes in air is an aerobic fungus, and enough fresh air is an important condition to ensure the normal growth and development of Lentinus edodes. Insufficient oxygen supply in the germ stage will hinder the respiration of the hyphae, maintain life temporarily with the help of glycolysis, increase nutrient consumption and weaken growth. Hypoxia in the mushroom emergence stage will affect the primordium differentiation and growth and development, resulting in deformities (long mushroom feet, large mushroom feet, deformed) mushrooms. Therefore, high-quality and high-yield Lentinus edodes can be obtained only in places with good ventilation.

5. Light hyphae can grow well under dark conditions, and strong light can inhibit the growth of mycelium. Under completely dark conditions, the fruiting body is not formed. If the light is insufficient, there are few mushrooms, long stalks, small flowers, light color and poor quality, but the strong direct light does great harm to Lentinus edodes, especially the young buds, so appropriate scattered light is very important for the development of fruiting bodies.

6. Like other wood rot fungi, Lentinus edodes pH likes to grow on acidic medium, and its pH is 5.5-6.5. In the suitable pH range, different pH had little effect on the mycelium growth of Lentinus edodes. In a word, the main factors of whether Lentinus edodes can successfully transfer from vegetative growth to reproductive growth and fruiting mushrooms are temperature difference stimulation, humidity and light. The key to further development after the formation of primordia is to ensure proper temperature and ventilation, as well as appropriate relative humidity and light. Only by simulating and creating the most suitable environmental conditions for the growth and development of Lentinus edodes can we obtain high yield and high quality.