The cost of cultivating Lentinus edodes with leaves is low.

Original seed culture is an important link in the cultivation of Lentinus edodes. The traditional method is to use sawdust, grain and other raw materials as culture medium. The preparation method is complex and needs to be carried out in a special culture room, and the cost is high. Using the fallen leaves in autumn as the main raw material, the composition of the culture medium is simpler and cheaper, and it can be carried out in ordinary rooms, which is beneficial to popularize among ordinary farmers.

First of all, the leaves (95%), gypsum powder (2.5%), sugar (1%), compound fertilizer (0.6%), urea (0.6%) and carbendazim (0.3%) of Platanus przewalskii (aka French sycamore) are compared according to the ratio in parentheses.

The sample was mixed evenly to make the original culture medium. It is appropriate to put the culture material into a plastic bag and add water to squeeze the culture material with your hand so that the hand is moist and no water drops flow down. After sealing the plastic bag, put it in a 30 ℃ environment and let it ferment for 7-9 days. When the fermented culture material is loaded into a 17cm × 30cm high-temperature polypropylene plastic bag to a height of 5cm, a wooden rod or glass rod is inserted vertically, the diameter of the rod is about 2cm. Continue loading to 20 cm, compaction, pull out the stick. Put a plastic ring on the mouth of the bag and seal it with cotton (or newspaper). The culture medium was sterilized with 0.1-0.12mpa for 20 minutes, cooled to room temperature, and inoculated under an alcohol lamp. (the pressure should not be reduced too quickly, so as not to burst the plastic bag.) when inoculating, put small pieces of potato mother seed into the small hole after the glass rod is removed, one at the mouth and one in the middle. After sealing, the cells were cultured under 20 ℃. After 40 to 50 days of growth, the mycelium grows in the whole bag and can be preserved or cultured.

If the sun-dried leaves were used as culture material, the mycelium grew slowly, and the time of mycelium full bag was 10 days behind that of fresh leaves, while the mycelium of Lentinus edodes cultured with fresh undried leaves grew faster. Therefore, ordinary farmers can directly use fresh fallen leaves without drying, and it is easy to operate. However, if the leaves are preserved, they need to be dried in the sun to prevent decay. The majority of farmers can make full use of the fallen leaves of Platanus acerifolia planted in central and northern China as a reliable raw material for culture medium. It can be seen that using autumn fallen leaves as the raw material of Lentinus edodes culture medium is simple and cheap and can be popularized among ordinary farmers.