Rapid Propagation of Anticancer Plant Hybrid Taxus chinensis

Hybrid Taxus mairei 8502 is one of more than ten cultivated strains of Taxus mandshurica Taxusmedia. Its rapid growth and wide adaptability are better than other strains and can be planted in most areas of our country. Taxusmedia is one of the yew approved by FDA to extract paclitaxel, an anticancer drug. The taxol content in the branches and leaves of the 5-year-old Taxus mairei was higher than that of the natural yew bark grown for 70 ~ 80 years. The taxol content of wild yew, including domestic varieties, is generally between 0.004 and 0.01%. The taxol content of Taxus mairei is 0.03-0.06%, which is 8-10 times that of natural yew, which can be planted in most parts of our country. In addition, the yew is evergreen all the year round and the autumn fruit is red, giving people a healthy and full appearance, resistance to pruning, good shape, and high horticultural value. Taxus is also popular as a shade-tolerant potted health plant indoors, which can not only purify the air but also pick leaves and make tea to prevent disease. Now the technology of rapid propagation of Taxus mairei

The introduction is as follows:

1. Nursery bed and substrate preparation can use nursery bed and burrowing tray to raise seedlings, but burrowing tray is the most advanced way to raise seedlings.

The main advantages of pot seedling cultivation are as follows: 1, effectively prevent disease infection; 2, keep moisture and fertilizer to take root quickly; 3, transplant at any time or @ # @ 136 seedlings, no need to slow seedling, transplanting does not discount.

Configuration of hole plate matrix: by volume percentage: grass peat 60%, vermiculite 20%, perlite 20%. Add 0.1% ferrous sulfate and zinc sulfate to dissolve in water and evenly. After mixing evenly, the film is covered with high temperature and stuffy for one week, and then it can be installed and ready for use.

II. Collection and treatment of cuttings

The young trees of 1-8 years old are the best female parents, and the more tender the branches are, the stronger the rooting potential is. 2-year-old branches can also be used, but the rooting time is prolonged by 20-30 days. The softwood cuttings with leaves can be carried out in spring, summer and autumn during the plant growing period. the rooting rate of the softwood cuttings before August is 89% after 35 days. In the experiment, 1-10 cm cuttings can take root normally, there is no difference, and strong seedlings can be formed if they are properly managed in the later stage. JH2 and ABT2 are the most effective rooting agents. The rapid growth of scale can directly soak the whole plant for 1 hour with JH2, saving more than 30% of labor.

III. Seedbed management

1. Always use a sunshade net with a light transmittance of 40-50% during seedling breeding. Taxus likes shade and shading is beneficial to growth and rooting.

2. The light-sensitive seedling automatic controller was used to control the automatic spray during the seedling raising period, and the spray interval of the strongest light was adjusted as follows: 2 minutes in the first 30 days, 4 minutes in the last 30 days, and 8 minutes after 60 days. Each effective spray time is set to 15-20 seconds.

3. Disinfect the seedbed and replenish the nutrition every 4 days.

IV. Seedling transplantation

The time of transplantation should be determined according to the method, environment and level of transplantation. in principle, it can be transplanted at any time after taking root. In the method of raising seedlings in sand bed, the seedlings were treated with controlled water 12 days before transplanting and were transplanted when the roots were aged and soft. When the root system reaches a certain length, when the seedling is not easy to be broken, it is the best to transplant. Sunshade net management is still needed after seedling transfer, and attention should be paid to watering and moisturizing within 2 weeks.