A New Technique for Anti-fouling of Mushroom in Bag Cultivation

Bag cultivation of Lentinus edodes is prone to pollution, in addition to complex technology, low level of production technology, simple conditions and other reasons, it is also related to raw materials, bacteria, the advantages and disadvantages of plastic bags, extensive production and management, single disinfection and control effect of long-term use of pure chemicals, drug resistance of miscellaneous bacteria and so on. In order to effectively solve the pollution problem:

The main results are as follows: 1. the culture medium can be prepared by conventional sawdust, wheat bran, cottonseed shell, corncob and crop straw. The ratio of material to water is about 1.0: (1.2-1.3), and the special antifouling agent is 0.4%-1.0%. When mixing the material, it should keep the same dosage or concentration according to the dosage ratio of the antifouling agent, dissolve it once or several times in the water of the mixture, fully dissolve and mix it evenly, then apply it into the evenly mixed dry culture material, and repeatedly pat the culture material with a shovel. Let the antifouling agent be fully immersed in the culture material, and when it is uniform, it can be bagged.

Second, bagging and sterilization should choose high-quality polyethylene or polypropylene plastic bags (cylinders) with more than 0.04mm toughness, uniform thickness, not easy brittleness and no leakage. During seed production, the material should be packed loose and tight moderately, and during cultivation, the material should be packed tightly, and the bag mouth should be packed to about 6cm or 10cm according to the flat material surface, and the bag mouth should be tightly fastened with plastic rope or sealed according to the conventional method. The general temperature reaches 100 ℃ and lasts for 4 hours and 8 hours (4 hours in small bags and 6 hours in large bags). If the plastic bag is larger and has more materials, the sterilization time can be appropriately extended for 2-4 hours. After sterilization, take out the culture bag and put it into the seed room to cool down to 25 ℃ or cool. Spray and disinfect the inoculation room evenly with 1.5% antifouling agent (which can also be sterilized by conventional methods). Inoculation can be carried out after 20 minutes.

3. In order to shorten the growth period and reduce the harm of diseases and insect pests, strong stress resistance, fast mushroom emergence and tide turning, short growth cycle, high yield, high quality and pollution-free bacteria should be selected for inoculation and culture. During inoculation, inoculation at one or both ends can be used to dig up the bacteria into blocks during the day, appropriately increase the amount of inoculation, promote the rapid growth of hyphae, shorten the fungus generation period, and improve the yield and quality of cultivation. After inoculation, it was transferred to the culture room for culture. The indoor room with better conditions such as heat preservation, ventilation and cooling should be selected as the culture room. After treatment with anti-insect and disinfectant before use, the inoculated culture bag was moved into a vertical or horizontal code on the indoor culture rack, and the temperature was kept at about 20-25 ℃. After about 10 days of culture, the bag should be turned over to check for survival and contamination. If there are unsurvived and contaminated culture bags, they should pick out the culture room, find out the cause, and take remedial measures (dig out the unsurvived and contaminated bacteria, make up inoculation after disinfection). During the training period, we should do a good job of heat preservation and moisturizing, cooling, transposition of bags, disinfection and insect prevention. When the mycelium is full of bags and then cultured for a period of time, the mycelium shrinks slightly and becomes compact, which can be used as a strain or for mushroom production management.

4. The small fruiting technique is most suitable for original seed cultivation and cultivation bag, and can also produce mother and original seed, and is suitable for seed production and cultivation of many kinds of edible mushrooms, such as Pleurotus ostreatus, Coprinus comatus, Pleurotus ostreatus, Pleurotus ostreatus and so on. If we can make full and rational use of the appropriate natural temperature and combine with artificial control measures, with the combination of high and low temperature type or alternating cultivation, weekly production can be formed. In production, as long as we master the ratio of material to water, fully mix the material, select high-quality plastic bags, maintain uniform concentration according to the amount of antifouling agent, and do a good job in disinfection and prevention, there is generally no pollution. If the culture material is fully mixed with 1% antifouling agent dissolved in the mixing water, it can be bagged, inoculated and cultured, eliminating the conventional link of bacteria and directly carrying out seed production and cultivation with raw materials. The dosage of disinfection and prevention and control of miscellaneous bacteria can be 1.5% and 2.0%. When using antifouling agent, it should not be lower than the minimum dosage, and there is no need to add other fungicides. If you need to enhance the control effect, you only need to increase the dosage appropriately.