The method of asexual propagation of Schisandra chinensis-hard branch splicing propagation

1-year-old shoots were collected as scions from defoliation to pre-sprouting. 1-2-year-old seedlings were used as rootstocks before freezing and stored at low temperature for cleavage in the sprouting period of the following year (or without local splicing of seedlings). Soak the scion and rootstock in clean water for 12 hours before grafting, and cut off the budding part at the Hypocotyl of the rootstock. The scion should choose a fully mature branch with a thickness greater than 0.4 cm, with a cutting length of 4 cm to 5 cm, leaving a bud hole, 1.5 cm above the bud and about 3 cm under the bud. Use the cutter to cut on both sides of the scion bud eye, the cutting surface is a wedge with a length of about 1 to 1.5 cm, and the cut scion is wrapped in a clean wet towel to prevent water loss. According to the length of the scion cutting surface, undefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefined cuts a split of about 2cm at the center of the rootstock, selects the scion with roughly the same thickness and inserts the scion into the split, requiring that one side of the scion is aligned, and the scion cutting surface generally retains 2mm "exposure", and then binds the whole interface tightly with plastic film. Transplant the grafted seedlings into the nursery according to the row spacing of 5 cm × 20 cm. In order to prevent the scion from losing water and drying up, the upper cut of the scion can be sealed with lead oil. Calli were produced in lO~15 days after transplanting and could germinate after 30 days. Shading should be carried out when about 30% of the grafted seedlings germinate, because the calli of the scion and the rootstock have not been fully combined at this time, and the water absorbed by the root system can not well supply the needs of the scion, which can prevent the scion from losing water and death caused by high temperature sunlight. Coring treatment is needed when the germinated shoots begin to elongate and grow. Generally, it is suitable to leave 2-3 leaves. When the temperature exceeds 30 ℃, the leaf temperature can be reduced by spraying water on the leaf surface to reduce leaf temperature and transpiration. When the sprouting secondary shoot begins to grow for the second time, it shows that it has been grafted and survived, and the shade can be removed.