Asexual Propagation and Seedling raising method of Schisandra chinensis-tissue Culture
As a new technology and scientific research means, plant tissue culture has developed vigorously in various fields of plant science, and has been widely used in agriculture, forestry, horticulture, Chinese herbal medicine and other production fields, showing great advantages. A large number of seedlings will be provided in a short time to meet the needs of production. As a means of asexual propagation, tissue culture can propagate 60 000 branches in 8 months with one apple bud, 1 million seedlings in one year with one poplar axillary bud and 500000 seedlings in one year with one bud of Schisandra chinensis. It can be seen that its application prospect is very broad.
The main results are as follows: (1) the axillary buds of Schisandra chinensis varieties or superior lines were used as tissue culture materials for the acquisition and treatment of explants. Cut 2 cm long tender stem segments with axillary buds, rinse them with water for 10 minutes, sterilize with 2% sodium hypochlorite for 20 minutes, rinse with sterile water for 5 times, then absorb water with aseptic gauze, and inoculate them on the medium for inducing axillary bud germination by aseptic operation.
(2) the medium for bud induction was MS+6-BA4 mg / L + IBA0.2 mg / L + LH1000 mg / L, vitamin doubling of MS, sucrose 30 g / L, Agar 9 g / L, pH 5.8.The temperature was 18 ~ 20 ℃, the light intensity was 800 ~ 1200 lux, and the bud could grow after 4 weeks.
(3) the buds up to 2 cm were cut from the branches and inoculated on the subculture medium of MS+6-BA4 mg / L + 1.BAO.2 mg / L + LH1000 mg / L. The vitamins of MS were doubled, sucrose 30g / L, Agar 9g / L, pH 5.8. the culture conditions were as follows: temperature 1820 ℃, light 10 hours, light intensity 800,1200 lux. After 4 weeks, the buds could grow, cut off and continue to propagate until the required number was reached.
(4) rooting culture transferred shoots larger than 2 cm to rooting medium: NH4N () s825mg / L, KN () 3950 mg / L, MgSO4 7H2 () 195mg / L, KH2PO41 () (] mg / L, F} EDTA35 mg / L, K10.83mg / L, HsBO36.2 mg / L, MnS (). 4H2O0.25 mg / L, CuSO4 5H2O0.025 mg / L, CoCl2. 6H2O0.025 mg / L, inositol 200mg / L, nicotinic acid 1.0mg / L, pyridoxine 1.0mg / L, thiamine 0.2mg / L, hydrolyzed albumin 100mg / L, indolebutyric acid 2mg / L, sucrose 15g / L, Agar 9g / L. Environmental conditions: the temperature is 20: 25 ℃, the light intensity is 2000 lux, the light intensity is 14 hours, it can take root after 4 weeks.
(5) several roots of more than 1 cm will grow and the root culture medium with whisker roots will be planted in a nutrition bowl of 8 cm in diameter, and the cultivation medium in the nutrition bowl will be sterilized humus soil. Keep relative humidity 85%, temperature 20: 25 ℃, light 2000-2800 lux. After 1 week, the relative humidity can be gradually reduced to natural conditions. After 4-5 weeks, when the seedlings grow new leaves and roots, they can be transplanted, and the survival rate is more than 90%.
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Harvesting and seed treatment techniques of Schisandra chinensis
The seeds of Schisandra chinensis have the characteristics of dormancy, the seed coat is hard and smooth, and the oil layer under the skin is not permeable and breathable; the naturally mature seeds have fine embryos and post-ripening characteristics. The longevity of the seeds without pulp is shorter, generally stored dry at room temperature for 2 months, the seed vigor decreases, and the shrunken rate of wild seeds is usually very high. there are two common colors of mature seeds, one is dark red, one is dark red, and the other is orange-red, which is easy to germinate. Schisandra chinensis generally enters the coloring period in late July, and the sugar pigment in the fruit increases with the fruit ripening.
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The method of asexual propagation of Schisandra chinensis-hard branch splicing propagation
1-year-old shoots were collected as scions from defoliation to pre-sprouting. 1-2-year-old seedlings were used as rootstocks before freezing and stored at low temperature for cleavage in the sprouting period of the following year (or without local splicing of seedlings). Soak the scion and rootstock in clean water for 12 hours before grafting, and cut off the budding part at the hypocotyls of the rootstock. The scion should choose a fully mature branch with a thickness greater than 0.4 cm, with a cutting length of 4 cm to 5 cm, leaving a bud hole, 1.5 cm on the bud and 3 cm under the bud; use a cutter under both sides of the bud eye of the scion
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