Discussion on extraction Technology of Schisandra Ointment

Hugan tablet has the effect of soothing the liver and regulating qi, invigorating the spleen and eliminating food, reducing serum alanine aminotransferase, and has a significant effect on chronic, viral hepatitis, persistent hepatitis and early liver cirrhosis. This product was originally collected by ministry standard and provincial standard, and was later included in the 95th edition of Chinese Pharmacopoeia. The detection item added "determination of Schisandrin B content", but there was no stipulation on the extraction process of Schisandra chinensis ointment. If Schisandra chinensis ointment is still extracted according to the raw material process, it is not safe to extract Schisandra chinensis B. For this reason, according to the properties of Schisandrin B, we designed the experiment of extracting Schisandra ointment with the same method with different concentration of ethanol and the same concentration of ethanol with different methods. Compared with the original method, the extraction is more complete. It is proved by experiments that the extraction of Schisandra ointment by 95% ethanol percolation is an ideal production process.

1 Materials, instruments and reagents

1.1 Schisandra chinensis (native Liaoning). After drying, crush it into fine powder.

1.2 the wavelength 254nm was determined by SP-8810-HPLC.

1.3 Reagent Schisandrin B (China Institute of Biological products).

2 Test methods, steps and results

2.1 take the same amount of Schisandra chinensis fine powder, refluxing and extracting twice with 75%, 80%, 95% ethanol respectively, the first time adding 4 times the amount of ethanol, refluxing for 3 hours, and the second time adding 3 times the amount of ethanol, refluxing for 2 hours. The extract was combined twice, ethanol was recovered under reduced pressure, and then concentrated to thick paste at atmospheric pressure, and its relative volume mass was about 1.028. Two kinds of thick ointments were weighed respectively and the contents were determined according to the method under the content determination of Hugan tablets in the first edition of Chinese Pharmacopoeia. The results are as follows.

Schisandra chinensis ointment is extracted with 75% ethanol, Schisandra chinensis B content is 0.242% Bing 80% ethanol extract Schisandra chinensis ointment, Schisandrin B content is 0.268% Bing 95% ethanol extract Schisandra chinensis B content is 0.317%.

2.2 take a portion of Schisandra chinensis powder and percolate it with 6 times the amount of 95% ethanol at a rate of per 3~4ml/min. The recovered ethanol was concentrated to thick ointment, and its relative volume and mass was about 1.028. The contents of two different methods of manuscript ointment were determined according to the first part of Chinese Pharmacopoeia to protect the liver. The result is:

The content of Schisandrin B extracted by 95% ethanol percolation method was 0.328%.

3 discussion

3.1 because the extraction method of Schisandra chinensis paste is not specified in the 95 edition of Chinese Pharmacopoeia, and the technological methods of the original part standard and provincial standard use 75% ethanol reflux to extract Schisandra chinensis paste. It is proved by experiments that the content of Schisandrin B is the highest when Schisandra ointment is extracted with 95% ethanol. Because Schisandrin B is a fat-soluble component, increasing the concentration of ethanol is beneficial to the complete extraction of Schisandrin B.

3.2 there is an oxygen ring in the structure of Schisandrin B. reflux extraction after heating for a long time will destroy its effective components and reduce its extraction content. Therefore, compared with the reflux method, the percolation method is more complete, which can reduce the damage to the effective components and increase the content.

3.3 the origin of Schisandra chinensis is not stipulated in the Pharmacopoeia. Because there is no Schisandrin B in the South Schisandra chinensis, only Schisandra chinensis can be used in Hugan tablets.

3.495% ethanol percolation extraction of Schisandra chinensis ointment is the best extraction condition for the production of liver protection tablets.