Seed production Technology of Edible Fungi

3. There are three methods for separating mother seed: spore ejection, tissue separation and matrix hyphae separation. This paper mainly introduces the tissue separation method. Tissue separation method: cut small pieces of tissue from each mushroom, transplant them to slant culture medium, and culture them into mother seeds. The fruiting body of edible fungi is actually the knot of binucleate hyphae, which has strong regeneration ability. Therefore, pure mycelium can be obtained by cutting a small piece of tissue, inoculating it on a suitable medium and culturing it at an appropriate temperature. Tissue separation is to select the mushroom into a sterile room, in the ultra-clean bench, with 75% alcohol cotton wipe cap and stalk, and then use flame sterilized scalpel in the middle of the stalk cut. Make the mushroom body open. At the junction of cap and stipe or pleat, cut a small piece of mushroom body with inoculation knife, then cut it into small slices of 5 mm ×10 mm longitudinally, pick a slice with inoculation needle, connect it to the center of slant culture medium, inoculate a small slice with each test tube, wait for its mycelium to germinate, and then obtain mother seed.

4. culture at proper temperature: putting the test tube into a sterilized incubator or culture room for culture at about 25 DEG C, so as to enable the spores or hyphae obtained by separation to develop at proper temperature. Two or three days after tissue isolation and inoculation, hyphae germinated and spread on the culture medium; after mushroom isolation and inoculation, the tissue pieces generally turned black and brown at first, and white velvety hyphae grew on the tissue culture pieces about 7 days later.

5. Purification of mother seed After mycelium germination, carefully observe, select pure color, healthy, normal growth and uninterrupted bacterial line, hook mycelium together with culture medium on the clean bench of sterile room, and connect to another prepared test tube culture medium. Under the constant temperature condition of 23 - 25 DEG C, culture for 7 - 10 days, observe after hyphae grow up in test tubes, and select the best from them, that is, the mother seed of "mother generation".

6. Transfer culture of mother seeds A tube full of hyphae was transferred to multiple tubes under aseptic operation. Generally, each parent seed can be expanded to 30 ~50, and most of the mother seeds supplied in production are progeny. It can be re-expanded. Generally, each branch can be expanded into 20 ~25 filial parent seeds, but the number of tube transfer should not exceed 4 times. Too many tube transfer generations tend to reduce the viability of the strain.

7. In fruiting experiment, mother seeds were inoculated on sawdust culture medium in bottle or bag, and cultured at appropriate temperature according to the temperature requirements of various mushroom species until fruiting was confirmed to be suitable for production.

II. Breeding and cultivation of original seeds

The mother seed is inoculated on a sawdust culture medium in a bottle or bag, and the mother seed is obtained by culturing.

III. Expanded cultivation of cultivated species

The original seed is re-inoculated on the same sawdust culture medium, and cultivated for 30 ~35 days under appropriate temperature conditions, which is the cultivated seed (also called production seed), which can be used for production cultivation.