Preparation of Mother Culture medium for Edible Fungi
1. 800 grams of corn, 20 grams of glucose, 20 grams of Agar, 1.5 liters of rice water. First, boil the corn kernels in dilute Amoy rice water, pick them up when a small amount of corn kernels burst and filter. Continue to heat the filtrate (if the filtrate is less than 1 liter, add clean water to make up), add Agar and glucose, stir until all the Agar dissolves, then separate the test tube, sterilize and place the bevel.
two。 Fresh Pleurotus ostreatus or mushroom foot 200 grams, glucose 20 grams, Agar 20 grams, water 1 liter. The preparation method is the same as potato glucose Agar medium.
3. Wheat bran 2pm 3, cornmeal 1pm 3, plus lime. Wet the wheat bran, cornmeal and lime with water, with a water content of 65%, then put it into a test tube and press it into a slope. This method is convenient and easy to make, and the mycelium grows vigorously after inoculation.
4. Cottonseed hull 80%, wheat bran 19%, lime 1%, mixed with water and then divided into test tubes (can be pressed bevel, this method has the advantages: first, it can be prepared at the same time when preparing the original seed and cultivated seed, without the need for additional raw materials; second, the mycelium grows well and is not easy to age.
5. Plane tree sawdust 90%, corn meal 9%, lime powder 1%, mix well with water and then separate into test tubes. The sawdust is about 3 mm square. This method is beneficial to the preservation of bacteria.
6. Each test tube is filled with 3 grams of rice and 4 milliliters of water, placed in a pressure cooker and sterilized at a pressure of 1.2 kg / square centimeter for 40 minutes. Or put it into a household pressure cooker and cover the pressure limiting valve when spraying steam from the orifice and maintain it for 50 minutes. Compared with the previous method, this method is simpler, germinates well and grows vigorously after inoculation, and it is easy to check whether it pollutes miscellaneous bacteria.
7. A certain number of grains of rice or wheat, well-cooked and not rotten. Remove and drain, add 1% lime powder, mix well and put into the test tube. This medium is nutritious, germinates well after transplanting the original seed, grows fast, and is not easy to be contaminated by miscellaneous bacteria.
The experiment shows that when a variety is expanded in the same formula medium, the superiority of the strain is easy to degenerate, thus affecting the yield and quality. The selection of different media for tube culture can better maintain the excellent characteristics of the strain, play a rejuvenation role to a certain extent, and lay a good foundation for the high quality and high yield of edible fungi.
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A simple method for making Mother species of Edible Fungi
PDA or PSA and their improved culture medium are mostly used in the production of edible mushroom mother seed, but in some remote areas or small batch production, the raw materials in the formula are difficult to be purchased in rural areas due to the high price (generally analytical purity) and delay production. This paper introduces several simple production methods. 1. Gelatin medium. Gelatin, which refers to edible gelatin, is sold in general food and chemical stores at a lower price. Gelatin used as a coagulant or solidifying agent in food production, instead of Agar (watercress, Agar) as coagulant in mother seed production.
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Key techniques of early Operation of Edible Fungi Culture material
It is very important to make a good culture material of edible fungus, which directly affects the growth of edible fungus and the benefit of growers. Here I will take Gymboree starter as an example to introduce the essentials of operation. 1. Preparation: according to 0.1% of the weight of the culture material (that is, 1/1000, if the newspaper waste is reused, 0.2% can be added) to prepare Gymboree edible mushroom culture material fermentation auxiliaries; pre-soak or pre-wet the crushed or cut-off main materials, adjust the moisture to 60%, 70%, and the PH value is about 8. The moisture content is too low, which is not conducive to the rapid propagation of functional microorganisms.
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