A simple method for making Mother species of Edible Fungi

PDA or PSA and their improved culture medium are mostly used in the production of edible mushroom mother seed, but in some remote areas or small batch production, the raw materials in the formula are difficult to be purchased in rural areas due to the high price (generally analytical purity) and delay production. This paper introduces several simple production methods. 1. Gelatin medium. Gelatin, which refers to edible gelatin, is sold in general food and chemical stores at a lower price. Gelatin, which is used as coagulant or solidifying agent in food production, is used in parent production instead of Agar (watercress, Agar) as coagulant. The author carried out experiments and used in production in the early 1990s, which proved that the effect was good. However, the coagulability test should be carried out on the goods purchased, and after the final determination of the amount used in every 1000 mm, the brand should be used and can not be replaced at will. The general production formula is: 200g of potato, 30g of wheat bran, 20g of sugar, 50g of gelatin and 1000 ml of water. 2. Raw grain type medium. That is, grain medium. @ #, corn, beans and so on can be used as mother culture medium, and the production effect is very good. The method is: corn and soybeans need to break ballast, make them similar in size to wheat grains, soak to no white heart, quickly rinse with cold water after being cooked in boiling water, fish out and then mix with appropriate amount of wheat bran, and then put into the test tube. It is suggested that 20 × 200 or 25 × 200 test tubes can be used to replace large test tubes when using this culture medium, which is not only convenient to operate, but also increase the quantity of one-time production, which is economical and economical in time. 3. Potato-shaped medium: select large smooth potatoes, peeled and cut into pout-shaped strips. The potato chips are 15 cm long, 13 cm and 0.2 cm thick at both ends, respectively, and the width is determined according to the diameter of the test tube, generally less than 2 cm, and try to make it semicircular, so that the round edge is close to the test tube wall. Loading into the test tube, routine sterilization and other operations can be done. 4. Pumpkin strip culture medium: after the pumpkin is ripe in autumn, it can be directly used as mother culture medium to cut pumpkin strips with reference to potato culture medium. 5. Apple paste medium: peel and core the ripe apples, grind the mud, put it into the test tube and sterilize it routinely. It is just that when inoculation is needed, the puree is pressed into a long strip with a sterilized wood strip, which is similar to the ordinary medium, and then routinely inoculated. 6. Carrot strip medium: it can be carried out with reference to potato medium.