Preservation of Mother species of Edible Fungi
The mycelium of edible fungi propagates quickly and is easy to mutate, so it is difficult to preserve the strains. This paper introduces four effective and simple methods.
1. Liquid paraffin preservation method: after high-pressure sterilization of liquid paraffin, put it in a thermostat for several hours or in a dryer for several days, absorb liquid paraffin with a sterile straw or syringe and inject it into the strain test tube. The injection amount should be about 1 cm higher than the tip of the inclined plane. Plug with cotton stopper, seal with wax, store upright in low temperature, can be preserved for 5 years.
Second, the preservation method of wheat bran, take a certain amount of wheat bran, add water or nutrient solution, mix well and then use. The wet wheat bran was loaded into the test tube, sterilized under high pressure and then cultured at the appropriate temperature. After the mycelium is well developed, the test tube is dried at room temperature. Store it in a dry place below 20 ℃ after drying, which can be preserved for 3 to 5 years.
3. The bacteria were inserted into potato culture medium by normal saline preservation method. 60ml culture medium was filled with 250ml triangle bottle, vibrated or shaken 5ml 10 times a day for 5ml for 7 days, then the mycelium ball was transferred into a test tube containing 5ml sterile saline. Each tube was filled with 4 to 5 mycelium balls, stuffed with cotton eggs and sealed with wax. At low temperature, it can be preserved for 1 year.
Fourth, the wheat grain preservation method takes the high quality @ # @ 245wheat grain, after washing, soaks in 20 ℃ water for 5 hours, slightly dries, installs the tube. The appropriate loading quantity is 1 hammer, 4 cycles, 1 stroke, 3 pipe length. Sterilize, shake while hot, cool and join the mycelium. Culture at suitable temperature, and the culture was terminated when sparse hyphae appeared on most wheat grains. Then, put it in a dry place below 25 ℃ and keep it for 1 to 2 years.
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Identification of Edible Fungi
Identification of edible fungi the mycelium of Pleurotus ostreatus is white, dense, stout, cotton-wool-like, and those with wall-climbing phenomenon are excellent strains. If the bacteria cultured with sawdust, the decomposed sawdust is light yellow, and a small amount of primordia appear, which is an excellent strain. If the mycelium is sparse or bundled, the cotton shell culture medium is dark red, the sawdust culture medium is white, the hyphae breaks away from the bottle wall and shrinks, there is yellow liquid accumulation at the base of the bottle, or mildew spots appear on the surface of the culture material, it is inferior bacteria. The mycelium of Auricularia auricula is white and neat, and it is an excellent strain. If
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Appearance Identification of Mother species of Edible Fungi
Excellent and purified strains are the fundamental guarantee for the successful cultivation of edible fungi. Several methods for identifying the mother species of edible fungi are introduced as follows: the mycelium of Lentinus edodes on potato glucose Agar (PDA) medium is pure white, stout, flocculent or fluffy, has a certain wall-climbing ability after full test tube, and the edge is irregular and wavy. At the temperature of about 24 ℃, the mycelium can cover the inclined surface of the test tube in 15 days. With the special fragrance of Lentinus edodes. The hyphae of Pleurotus ostreatus were white, dense and stout on PDA medium.
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