MySheen

Preservation of Mother species of Edible Fungi

Published: 2024-11-08 Author: mysheen
Last Updated: 2024/11/08, The mycelium of edible fungi propagates quickly and is easy to mutate, so it is difficult to preserve the strains. This paper introduces four effective and simple methods. 1. Liquid paraffin preservation method: after high-pressure sterilization of liquid paraffin, put it in a thermostat for several hours or in a dryer for several days, absorb liquid paraffin with a sterile straw or syringe and inject it into the strain test tube. The injection amount should be about 1 cm higher than the tip of the inclined plane. Plug with cotton stopper, seal with wax, store upright in low temperature, can be preserved for 5 years. 2. weigh a certain amount of wheat bran by preservation method, add water or nutrient solution and mix well.

The mycelium of edible fungi propagates quickly and is easy to mutate, so it is difficult to preserve the strains. This paper introduces four effective and simple methods.

1. Liquid paraffin preservation method: after high-pressure sterilization of liquid paraffin, put it in a thermostat for several hours or in a dryer for several days, absorb liquid paraffin with a sterile straw or syringe and inject it into the strain test tube. The injection amount should be about 1 cm higher than the tip of the inclined plane. Plug with cotton stopper, seal with wax, store upright in low temperature, can be preserved for 5 years.

Second, the preservation method of wheat bran, take a certain amount of wheat bran, add water or nutrient solution, mix well and then use. The wet wheat bran was loaded into the test tube, sterilized under high pressure and then cultured at the appropriate temperature. After the mycelium is well developed, the test tube is dried at room temperature. Store it in a dry place below 20 ℃ after drying, which can be preserved for 3 to 5 years.

3. The bacteria were inserted into potato culture medium by normal saline preservation method. 60ml culture medium was filled with 250ml triangle bottle, vibrated or shaken 5ml 10 times a day for 5ml for 7 days, then the mycelium ball was transferred into a test tube containing 5ml sterile saline. Each tube was filled with 4 to 5 mycelium balls, stuffed with cotton eggs and sealed with wax. At low temperature, it can be preserved for 1 year.

Fourth, the wheat grain preservation method takes the high quality @ # @ 245wheat grain, after washing, soaks in 20 ℃ water for 5 hours, slightly dries, installs the tube. The appropriate loading quantity is 1 hammer, 4 cycles, 1 stroke, 3 pipe length. Sterilize, shake while hot, cool and join the mycelium. Culture at suitable temperature, and the culture was terminated when sparse hyphae appeared on most wheat grains. Then, put it in a dry place below 25 ℃ and keep it for 1 to 2 years.

 
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