Disease Ganoderma lucidum yeast

Yeast is a kind of ascomycetes without filamentous mycelium structure, which is widely distributed in nature. Ganoderma lucidum, Tremella fuciformis and other edible fungi can pollute the culture medium and culture materials in the production and cultivation process, mainly polluting the culture materials, leading to the deterioration of the culture materials and inhibiting the growth of hyphae.

The infection of symptomatic yeast usually starts from the middle of Tremella culture material, which leads to fermentation deterioration and the smell of alcoholic acid, which affects the growth and development of hyphae, and then affects its yield and quality.

The pathogen belongs to ascomycetes. The colonies grown on potato sucrose Agar medium are paste or colloid with milky white, pink and milky yellow colors. Some are sticky, some have neat edges, some are smooth, and some are wrinkled. The spores were observed under the microscope, the spores were colorless and single-celled, similar to the morphology of mother cells.

The characteristics of the disease are that yeast lives on organic matter and spores spread with airflow. Due to incomplete disinfection and sterilization in the process of strain production or raw material cultivation, the bacteria is easy to occur because there are many and tight materials in bottle bags, and the discharge between bottles and bags is tight. High temperature and humidity are beneficial to the germination and reproduction of yeast. too thick bed material and high water content of culture material will lead to fermentation deterioration of culture material.

Prevention and cure method

The main results are as follows: (1) in the production of bacteria, an appropriate amount of strain bags should be used to avoid too tight bags of culture materials. During sterilization, keep a proper distance between the bottles and bags, which is conducive to neat evacuation. The original species and cultivated species should not be sterilized by intermittent sterilization under atmospheric pressure. Sterilization at atmospheric pressure should be kept at least 100 ℃ for 8 hours.

(2) when aseptic operation is carried out during vaccination, the staff should strictly abide by the vaccination procedures.

(3) in the process of cultivation, 25% carbendazim or 0.1% methyl topiramate was mixed with 0.2% of the dry material weight. Keep the appropriate water content and temperature of the culture material. Spray with clean water to reduce the occurrence of germs.

(4) in the cultivation and production, it is found that the temperature of the culture material is too high and gives off a sour taste, and the culture material is irrigated with lime water (pH=13-14) in time to control the propagation of yeast.

Commonly used agents carbendazim, methyl topiramate.