The inoculation account is good for inoculation of edible fungi.

1. Make it. The mosquito tent type plastic inoculation shed is made of agricultural plastic film with a width of 3 meters and a thickness of 0.5 mm. The specifications of the inoculation shed are 3 meters long, 3 meters wide and 3 meters high. First, the 13.5 m long plastic film was cut as the 3 m square wall of the inoculation shed, and the 3 m long plastic film was taken as the roof. Then seal the two films along the edge with a plastic sealing machine or electric soldering iron, leaving a masked connection line on one side of the shed as the door of the inoculation shed, and tie a rope at each of the four top corners of the inoculation shed to facilitate hanging.

two。 Disinfect. Clean the inoculation site before use. Change the height of the shed to 2.5 meters, and the remaining 0.5 meters fold into the shed. Then the cooled bacterial stick is moved into the shed, stacked along the shed wall, and the plastic shed is partially folded and compacted. Leave a space in the shed for the seed table and the staff to move about. Before disinfection, put the bacteria, cultivation bags and thousands of inoculations in the inoculation account in advance. After preparation, close the inoculation shed for disinfection. The use of aerosol disinfection box, 4 grams per square meter, 50 grams per box, 15 square meters of inoculation account with 2 boxes. Ignite the disinfection box and disinfect it airtight for 7 hours for 8 hours to achieve the sterilization effect. In addition, vaccinators should wear work clothes and work caps, keep their personal hygiene clean before entering the account, trim their nails, and disinfect them in a pre-prepared 5%-10% cresol soap solution. The arms should be soaked in bromogeramine 50 times solution for 5 minutes or wiped with 75% alcohol cotton. Leave the shoes outside the account when you get the money, and then sterilize the vaccination tools with 75% alcohol cotton.

3. Vaccination. The method of double bag inoculation was used. In the double-bag method, the bacterial bag is made by filling the bag with a bagging machine, and then cover the outer bag with a layer of outer bag. when inoculating, unlock the outer bag and punch 5 or 4 holes, the hole depth is 2-3 cm, insert the strain block into the hole, then tie up the outer bag immediately, and the inoculation is completed. During inoculation, the inoculation tools were wiped with 75% alcohol and sterilized by flame, sterilized once at a time, and the outer wall and mouth of the strain bottle were sterilized with 0.1% potassium permanganate aqueous solution. The inoculation tent has built-in alcohol lamp, and when inoculating, the mouth of the strain bottle and the inoculation mouth of the bacteria bag are in the sterile area of the alcohol lamp flame, and the movement of the strain block should be quick. Each bottle of 450 ml or 500 ml bacteria can be inoculated with 10-15 bags at both ends. The size of broad bean is suitable for the strain block. Vaccination operation should be continuous operation, quick release of bag mouth, quick inoculation, quick opening, quick placement and proper tightness. For good ventilation, the sterilized sponge can be put into the mouth. A vaccination account put about 1000 bags at a time, it should be picked up within 4-5 hours, usually in groups of three, with cooperation. After inoculation, the bacterial rods can be cultured on the spot, and the bacterial bags after inoculation overlap according to the shape of "well", with 3 bags in each row and 10 layers. The temperature in the greenhouse was maintained at 22: 26 ℃. The greenhouse was covered with grass curtains to prevent strong light, and the shed was not removed until the head of the hyphae reached 5: 10 cm. There are also cases where the bags are shipped out and sterilized separately. After each inoculation, it is necessary to clean up tools and sundries, clean up, and fumigate once. The amount of formaldehyde is 10 to 15 milliliters per cubic meter, and then inoculate.

4. Advantages. Compared with the inoculation room (box), the inoculation shed has the advantages of low cost and high labor efficiency. The production of a mosquito tent vaccination shed costs only more than 30 yuan, which is 1 / 5 of the inoculation box, and it is simple to make and can be used continuously for 3 years. The inoculation box receives about 25 bacteria sticks per hour, and the inoculation shed can reach more than 50 per hour. Moreover, the bacteria are cultured on the spot and no longer sealed with other things, thus saving labor.