High-yielding techniques of Ganoderma lucidum cultivated in bottles at Home

Ganoderma lucidum is a precious medicinal fungus. Through the ages, Chinese and foreign medical experts believe that it has the effects of strengthening the foundation, strengthening the heart and strengthening the brain, and has a certain curative effect on diseases such as hypertension. At present, the new anti-cancer drug of Ganoderma lucidum wall-breaking spore powder pushes this magical fungus to a new peak in pharmacology.

Due to the scarcity of wild Ganoderma lucidum, it can not meet the needs of people. If you grow this magic fungus in a bottle in your own courtyard, it will satisfy people's curiosity.

First, the growth and development conditions of Ganoderma lucidum. Ganoderma lucidum is a high-temperature fungus. The hyphae can grow between 12 and 36 ℃, and the fastest growth is at 27 ℃. The fruiting bodies could differentiate between 18 and 32 ℃, but the normal cap and fruiting bodies could be formed when the temperature was above 20 ℃. It is sensitive to light, scattering light is needed when the fruiting body occurs, and the air should be kept fresh. The pH value grows best between 5 and 6.

Second, the preparation of culture medium and the selection of cultivation bottles. Bottle cultivation of Ganoderma lucidum generally uses 750 ml wide-mouth bottles with a caliber of 3 cm, and families can sometimes use 500 ml canned bottles, it is best to choose a smaller caliber. Bottle-planted Ganoderma lucidum is generally produced directly by cultivated species. The main purpose is to prevent man-made pollution as much as possible when sowing. Medium composition: fresh cottonseed shell of the same year, exposed for 2-3 days, 50 kg cottonseed shell, 1 kg calcium superphosphate, 1-1.5 kg gypsum powder, 1 kg quicklime to adjust pH to 5-6. According to the ratio of material to water 1: 1.4, that is, the water content is 65%, hold the well-mixed material tightly by hand, and there are 1-2 dripping water between the fingers, then fill the bottle in time, and compact the culture medium in the bottle with a small stick. In order to facilitate inoculation, after filling the bottle, use a small stick to pound a small hole from top to bottom in the center of the bottle, then wash the sundries at the mouth of the bottle, close the mouth of the bottle with a clean cotton stopper, wrap it with moistureproof paper, put it in a 1.5 kg / square centimeter high-pressure steamer for 1 hour, unconditionally cover a steaming bucket with an atmospheric pan, boil continuously for 10 hours, and simmer for 1 day.

Third, vaccination. Put the sterilized bottles into the inoculation box one by one. Half an hour before inoculation, disinfect the box with formaldehyde and potassium permanganate solution; when inoculating, people's hands and arms are disinfected with alcohol and inoculated in the inoculation box. Open the cotton stopper, use sterilized tweezers to pick up the original broad bean seed and put it into the inoculation hole, then seal the cotton plug. After all the bottles in the inoculation box have been inoculated, take out the bottles.

Fourth, mycelium culture. According to the demand of mycelium growth, a dry, clean and pollution-free room was selected as far as possible for mycelium culture, and the humidity was maintained at about 25 ℃. Pay attention to ventilation and cooling in summer. Check for miscellaneous bacteria pollution every day. Generally, there are green or yellowish-green hyphae in bottles contaminated by miscellaneous bacteria, which should be eliminated in time. When the mycelium was cultured for about 20 days, the buds appeared on the surface of the culture medium, and the cotton plug could be loosened to facilitate ventilation and accelerate the growth of the stalk. At this time, the air humidity should be increased to 80-90%, but do not spray water directly to the mouth of the bacteria bottle.

Fifth, the growth stage of bacterial cap. At this stage, the top of the stalk expands, forming a flaky cap with a wheel growing along the horizontal direction.

Sixth, the harvest stage. When the color of the cap changes from milky white to beige, and then from beige to reddish brown, the spores of the seed layer have also matured. At this time, you can use a sharp knife along the bottom of the stalk to cut off, and clean up the remaining mycorrhiza in time, try not to hurt the mycelium, cover the wet newspaper, and re-enter the mycelium growth recovery stage for the second time.

Ganoderma lucidum needs scattered light in the stage of Ganoderma lucidum. If there is only a stalk but no cap, it is often caused by insufficient light.