MySheen

Propagation methods of Taxus mairei

Published: 2024-11-22 Author: mysheen
Last Updated: 2024/11/22, 1. Seed propagation: select the wet and thick dark brown soil on the shady slope and sow in autumn and spring. The seeds without aril were treated with wet sand stratification for 19 months from November to April, the seed embryos turned yellow and the seeds germinated. The aril was removed and soaked in 100ug/g 920 for 1 hour, and the wet sand layer could germinate for half a year. Cutting propagation: use vegetable garden soil, sand and charcoal ash to mix and build a shade shed with a transmittance of 30, cover with film to moisturize. First cut the one-year-old branches into 10~15cm cuttings, and the cuttings should be cut with 10.

1. Seed propagation: selecting moist and thick dark brown soil on shady slope, sprouting and drilling in autumn and spring, seeds without aril were treated by wet sand stratification for 19 months from November to April of the third year, embryo turned yellow, seeds germinated and cracked, aril was removed, soaked in 920 for 1 hour at 100ug/g, seeds germinated after wet sand stratification for half a year.

2. Cuttings propagation: vegetable soil, sand mixed with charcoal ash and light transmittance of 30 shade, cover film moisture. The cuttings were first cut into 10~15cm cuttings, disinfected with 1000 times 50 formalin for 12 hours, treated with 500 ppm ATP rooting powder, and planted on seedbeds. After 70 days, the rooting rate was 83. The cuttings were treated with 100ppm IAA and rooted in vermiculite for 45 days. From May to June, the cuttings were soaked in 100ppm IAA or 100ppm NAA for 3 hours, and then planted on the seedbed according to the plant spacing of 6×10cm. The cuttings were watered and kept moist by film for 50 days. The seedlings could be harvested in the third year and planted according to the plant spacing of 100 cm. The leaves could be harvested in 3 ~5 years.

3. Tissue culture: Roots were induced in 2~5mg/L NAA +0.2 mg/L KtB5 medium, embryoid was cultured in 5mg/L zip +5 mg IBA B5 medium (16h illumination). The germination rate of immature or mature embryos cultured on Wohite and Ms medium was 70. The germination rate was 80 when treated at 4℃. The growth of roots and embryos was promoted when MgSO 4 was added. Embryos were cultured on MS or B 5 medium with GA3kt10mg/L to mature. Complete plants were induced on N 6 or white medium containing 1% sucrose.

 
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