Propagation methods and techniques of Taxus mairei
For propagation of Taxus chinensis, good soil plots should be selected first, such as loose, humus-rich, neutral or slightly acidic alpine platforms, deep humid brown earth and dark brown earth on both sides of valleys and streams. Deep ploughing, leveling, according to plant spacing 0.4 m ×1.0 m or 0.4 m ×0.4 m hole, hole depth 40 cm, ready for planting.
The propagation methods of Taxus chinensis include seed propagation and cuttage propagation, mainly seedling transplantation.
1. Seed breeding
1. Seed picking and germination
In mid-to-late October, the seeds are harvested when the yew fruit is dark red. The seeds are physiologically post-mature and need to be stored in wet sand for one year to germinate. In order to improve germination rate, the germination of Taxus chinensis seeds is often treated by stratification method of outdoor natural wet sand storage.
2. Selection of nursery land
It is appropriate to select paddy fields with deep soil layer, loose structure, rich organic matter, good drainage and no exposure to the west (the paddy soil must be harvested in the same year). After harvesting the middle and late rice, the soil is dried, deeply turned once, crushed once, and then divided into compartments to make beds. Bed width 1.5 m (net bed width 1.2 m, footpath width 0.3 m); bed height 20 cm, seedbed length depends on nursery land.
3. Fertilization of nursery land
While preparing the soil for bed preparation, about 8000 jin of decomposed pig dung and 200 jin of instant high-quality compound fertilizer are applied per mu, and 6 to 8 jin of furadan are mixed to prevent underground pests. After turning the fertilizer into the soil layer and leveling the compartment surface, fill the compartment surface with yellow subsoil to a thickness of 5 cm.
4. Timely sowing
Taxus seed sowing points broadcast and drill two kinds: broadcast is to spread Taxus seeds evenly on the seedbed filled with yellow soil, cover the seeds with yellow soil, to see the seeds for the degree, and then cover the seedbed with moss. For drilling, 15 cm wide planks are selected and the seed beds filled with yellow subsoil are pressed into furrows 2 cm deep, with distances between furrows 15 to 20 cm. The seeds of Taxus chinensis are evenly sown in the ditch, covered with yellow soil, the thickness of which is 1 cm, and then covered with moss.
It is usually planted in early spring. After storage for one year, when 30% of the seeds are cracked and white, the seeds are screened out in time, disinfected in 0.2% potassium permanganate solution for 10 minutes, washed with clean water, dried and sown evenly in the ditch. Sowing is dominant, grain spacing 5 - 7 cm. Spreading can also be used. After sowing, dig yellow loam soil with mycorrhizal fungi and sieve under pine forest to cover seeds, and the thickness is less than that without seeds.
It should be noted that: first, strictly control the seeding rate, 15 kg per mu, otherwise it will seriously affect the qualified emergence rate of biennial yew seedlings; second, after sowing, the seeding bed must be covered with moss, and the seeding trench should be covered with moss. After the seedlings are unearthed, do not remove the moss and keep it on the seedbed for a long time.
5. Seedling management
The seedling rate of Taxus chinensis seeds is above 70%. Taxus seedlings pay attention to shade, seeding covered with straw to see no soil is appropriate, seedling set up shade, transmittance of 60%. Then spread moss to protect seedlings, protect the seedbed from sun and rain, and always keep the soil loose and moist.
The advantages of moss seedling protection are: protecting the seedbed from rain splash and direct sunlight, preventing the seedlings from being burned by sunlight and from suffocation and death due to the formation of mud sticks on stems and leaves, and often keeping the soil loose and moist, reducing the labor of intertillage and weeding. But the moss cover should be thin, not too thick, such as in case of drought without rain, available fine loess pressure moss, or spray water with a sprayer to keep the seedbed moist.
II. Cutting seedlings
During the dormancy and germination period of Taxus chinensis, the mixed substrate of sand, sawdust and pearlite was selected as cuttage soil. 1 - 4 year old yew lignification seedlings are selected, cuttings are cut into small sections of 10 cm, 15 cm or 30 cm long, and the cuts are required to be smooth, the lower cut is shaped like a horse ear, and leaves are removed below 2/3. The survival rate of cuttings was above 85% when treated with ATP, ABT, NAA, IBAA and so on. Pay attention to keep warm at seedling stage and build low shed for shade. Transplanted the following year.
From May to June every year, the semi-lignified branches of Taxus chinensis in the current year are cut as cuttings, and the mother trees are selected to be less than 10 years old. 15 - 20cm in length, cut off lower branchlets and needles into horseshoe shape, soak in ABT7 rooting powder with concentration of 30×10-6 - 50×10-6 for 3 - 12h. The cuttings were then inserted into seedbeds with river sand as substrate. Seedbed requirements 1 m wide, suitable length, cutting depth 3~5 cm. The spacing between rows and plants is 10cm×8cm, and water is sprayed immediately. The seedbed is set up and covered with plastic cloth. Water is sprayed 2~3 times a day, and 1 time a day after half a month. Generally, the ground temperature is maintained at 20~30℃, and the roots can be rooted after 30~40 days without strong light irradiation. During this period, foliar fertilizers such as brassin can be sprayed to promote growth.
When yew seedlings take root, fine management to promote vigorous growth, and appropriate ventilation and light training seedlings, increase resistance. The seedlings can be transplanted on a large seedbed after the end of the training.
Taxus seedlings generally grow in seedbeds for 2 to 3 years, and are regularly sprayed with auxin and foliar fertilizer every year. When the seedling height is 50 - 100 cm, it can be planted in pieces on the mountain. Planting time for late autumn or spring can be, plant spacing can be 3 meters ×4 meters, can also be in the streets, parks, for ornamental tree planting.
III. Transplanting
General yew seed seedling 1~2 years, cutting propagation of about 1 year, when yew seedlings grow to 30-50 cm high can be transplanted. Transplanting in October to November or February to March before germination, planting 1 seedling per hole, watering, appropriate shade.
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Rapid Cuttage Propagation of Taxus chinensis
Taxus is a national first-class protected plant, which is an evergreen tree, mostly distributed in the Northern Hemisphere. Its bark, stem, leaf and root can extract paclitaxel, which has a special effect on the treatment of cancer. We successfully introduced Taxus in the spring of 1996. The techniques of cutting propagation are briefly introduced as follows: first, during the cutting period, Taxus chinensis can be propagated three times a year in March, July and November, respectively. Second, to build a cutting bed, if you want to cut a small amount of yew, you can use pot cuttings, and if you have a large amount, you should build a cutting bed. Cuttage
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Propagation methods of Taxus mairei
1. Seed propagation: select the wet and thick dark brown soil on the shady slope and sow in autumn and spring. The seeds without aril were treated with wet sand stratification for 19 months from November to April, the seed embryos turned yellow and the seeds germinated. The aril was removed and soaked in 100ug/g 920 for 1 hour, and the wet sand layer could germinate for half a year. Cutting propagation: use vegetable garden soil, sand and charcoal ash to mix and build a shade shed with a transmittance of 30, cover with film to moisturize. First cut the one-year-old branches into 10~15cm cuttings, and the cuttings should be cut with 10.
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