Immunization methods for various diseases of pigs
I. immune method of rotavirus infection
1. Attenuated vaccine. The attenuated strain of pig origin was developed in China, and the cell passage was weakened, and the incidence of diarrhea in piglets born to immunized sows could be reduced by more than 60%.
two。 Fire fighting vaccine. Developed in China, the protective rate of this vaccine against piglets is more than 90%.
Second, the immune method of vesicular disease
China's development, including attenuated live vaccine and inactivated vaccine. Inactivated vaccines have been mainly promoted in recent years.
1. Live attenuated vaccine. Porcine vesicular disease rodent attenuated vaccine.
The protection rate is more than 80%, and the immune duration can reach half a year. The disadvantage is that the output is low and is not suitable for large-scale factory production.
two。 Porcine vesicular disease cell culture attenuated vaccine.
The protection rate was more than 80%, and the immunization period was 4 to 5 months. This kind of vaccine has high yield, good immunogenicity, safety and is suitable for large-scale industrial production.
III. Immunization methods of inactivated vaccine
1. Tissue inactivated vaccine against porcine vesicular disease in hamsters.
In order to infect hamsters with virulent virus, the infected tissue was inactivated and made with aluminum glue adjuvant. After injection of 2Ml/, immunity was produced in 7 days, the protection rate was more than 80%, and the immunization period was about half a year. The vaccine is safe and has no adverse reactions. 4 degrees can be preserved for 8 to 9 months. Anti-freezing.
two。 Cell culture inactivated vaccine against porcine vesicular disease.
It is made of refueling adjuvant for virus inactivation in primary hamster kidney cells and passage cells. The protection rate of effective inspection is 100%. When 3mL/ was injected intramuscularly, the immune effect was better twice a year, and the immune interval was two months.
IV. Immune methods of foot-and-mouth disease
At present, inactivated vaccines are mainly used. For example, the protective rate of BEI inactivated oil adjuvant vaccine against porcine type O foot-and-mouth disease was more than 90%, and the immune period was 9 months 10 days after injection. In addition, there is a concentrated inactivated vaccine, which is expensive and not widely used.
5. Immunization methods of porcine lung disease
1. Attenuated vaccine of porcine lung disease 670 to 230.
Freeze-dried vaccine. For oral use, injection is strictly prohibited. The immunization period was 10 months.
two。 Porcine lung disease C20 attenuated vaccine.
Freeze-dried vaccine is also used orally.
3. Attenuated EO630 vaccine of porcine pulmonary disease.
Freeze-dried vaccine, 20% aluminum hinge saline dilution, subcutaneous injection of 1Ml.
In addition, there is porcine lung disease Inner Mongolia attenuated vaccine, oral immunity.
The above live vaccines are used to prevent porcine lung disease caused by Pasteurella multocida.
VI. Methods of immunization against streptococcosis
1. At present, inactivated vaccines are mainly used.
Aluminum glue adjuvant in situ inactivated vaccine.
Oil adjuvant inactivated vaccine in the field.
The immune effect of oil vaccine is better than that of aluminum gel vaccine. The local occurrence of the disease can be controlled. Intramuscular injection, the immunization period is about half a year.
two。 Attenuated live vaccine.
For example, the attenuated strains of ST171 and G10S115, in which ST171 is widely used, the protective rate of freeze-dried vaccine is more than 75%, and the immunity period is 6 months.
7. Immune method of porcine atrophic rhinitis
Inactivated vaccine is mainly used. The effect of oil adjuvant vaccine is better. In general, the sows were vaccinated twice before delivery, and the piglets were vaccinated once at the age of 1 week and 3 to 4 weeks, and once again at 3 to 6 months. There is also an inactivated oil adjuvant vaccine against Bordetella-Pasteurella multocida.
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Diagnosis and treatment of Porcine dysentery (Ⅱ)
4Laboratory examination 4.1 No bacteria were found in the smears of heart, blood, liver and spleen of dead pigs, Gram staining and Wright's staining, and microscopic examination. 4.2 the mucus and bloody feces of acute diseased pigs were taken and the same amount of normal saline was added to make suspension. When the suspension was examined by dark field microscope, 3-5 spirochetes with consistent shape, sharp ends and serpentine activity could be seen in multiple visual fields. 5 Prevention and treatment of 5.1 whole herd pigs selected Shijing (3-methyl-2-acetyl-quinoline-1, 4-2)
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Diagnosis and treatment of chicken enterotoxin syndrome
1. The main manifestations of enterotoxin syndrome. Decreased feed intake, reduced weight gain or weight loss. The feces become thin and shapeless, with undigested feed in the feces, and the color becomes pale yellow, yellow-white or fish-intestine-like feces or carrot-like feces. Sudden death before dawn increased significantly or excited first and then limp, exhaustion death. Most of them are about 40 days old. In broiler flocks, whether raised on the ground or online, it is common, resulting in huge hidden economic losses, which are often caused by breeders and veterinary workers.
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