Ramet propagation of Cartland

Cartland is usually selected and propagated, usually in the spring and autumn rainy season. The rhizome at the base of it can be carefully cut with a knife, and each clump should have 2 pseudobulbs with new buds.

How does Cartland reproduce, divide and cultivate aseptically?

Cartland commonly uses ramet propagation methods for reproduction, and some flower friends use aseptic breeding methods. Here are Cartland's ramet and aseptic breeding methods and steps.

How to reproduce Cartland 1. Ramet propagation method

Cartland ramet time. You can choose to split plants during the spring and autumn rainy season. Flower friends can first use sharp cutting tools to cut the roots at the base of Cartland and cut them off for disinfection. Each clump needs at least two pseudobulbs with new buds, and then plant the new buds in pots.

2. Aseptic breeding

First of all, the ripe fruit should be selected and soaked in 75% alcohol for half a minute, then sterilized, put the fruit in 0.1% mercury solution, soaked for 20 minutes, then washed with sterile water for 4 or 5 times, and then cut open, the white powdery seeds were inoculated into the seed germination liquid culture medium, and the 150rpm shaker vibrated.

Generally, the embryo germinates after a week, and the green protocorm with a diameter of 1-2mm is formed in 20 days.

The protocorm was inoculated on the seedling culture base with a straw, and a complete plantlet could be formed in one month, and it could grow into a strong and healthy seedling for 45 days.

When the test-tube plantlets could grow to 3 cm high and 4 cm high, they were transferred to natural light for 1-2 weeks, and the investigated plants formed a better stratum corneum. After taking it out of the glass bottle, wash the root culture base, move it into the mixed pond pillar and carbonized bark matrix, adhere to the appropriate humidity, and do a good job of ventilation.

How does Cartland reproduce?

Ramet propagation of Cartland

The ramet method is relatively simple to operate and can be used by ordinary families. It is suitable to choose the rainy season in spring and autumn to carry out ramet work. Use a sharp cutting tool to cut the root between the two "orchid heads", carefully cut the rhizome at the base, and disinfect the incision. Each clump requires at least 2 pseudobulbs with new buds.

Aseptic seeding and tissue Culture of Cartland

In the early stage, liquid culture is mainly used to promote the rapid germination of seeds, and then the protocorm can be directly inoculated on the culture medium to achieve one-step seedling formation, the specific steps are as follows:

Materials

The healthy Cartland mother plant was selected for self-pollination and hybridization during flowering, and the fruit could be basically matured about 120-150 days after pollination.

Aseptic sowing

Select the ripe fruit, soak the fruit with 75% alcohol for half a minute, then disinfect the fruit, put the fruit in 0.1% mercury solution, soak for 20 minutes, rinse the fruit with sterile water for 4 or 5 times, then cut the fruit, inoculate the white powdery seeds in the seed germination liquid medium, and shake the culture in 150rpm shaker. A week later, the embryo germinated and a green protocorm with a diameter of 1-2mm was formed in 20 days.

Seedling culture

When the protocorms were inoculated on the seedling culture medium with a straw, the complete plantlets could be formed in one month, and the seedlings could grow into strong seedlings after further culture for 45 days.

Transplanting of test tube plantlets

When the test-tube plantlets can grow to 3pm and 4cm high, they were transferred to natural light for 1-2 weeks to observe the formation of better cuticular layer. After taking it out of the glass bottle, wash the root culture medium and move it into the cornerstone of the mixing pond and carbonized bark matrix to maintain proper humidity and ventilation.