Is Chinese rose grafted seedling or cutting seedling better? Analysis on the advantage of Rose grafting Cuttage

The day before yesterday, the editor talked about the technique of twisting branches and promoting bamboo shoots of rose, and got a lot of feedback from flower friends. One of them asked an interesting question. She asked, "can grafting promote bamboo shoots?" This question seems to be irrelevant, because grafting is only a way of reproduction, and the explosion of bamboo shoots is a growth process, and there is no overlap between the two, but there must be a reason why this flower friend can ask such a question. Her rose grafted seedlings grow bamboo shoots, and they grow from the ground. So when she saw the skill of twisting roses, she thought of her own rose flowers.

Today, the editor will talk about the difference between rose grafted seedlings and cutting seedlings, as well as the matters needing attention in purchasing grafted seedlings.

Flower friends often ask the editor, is the rose good for cutting or grafting? Because some people say that the grafting rose is good, some people say that the cutting rose seedling is good, so many people foolishly do not know. In fact, between rose cutting seedlings and grafted seedlings, can not simply say who is better, who is worse, can only say that each has its own advantages! Only by understanding the respective characteristics and needs of cutting rose seedlings and grafted rose seedlings, can we make the best choice.

1. Characteristics of grafted seedlings:

For many excellent rose varieties, cutting propagation is not easy to survive, so grafting propagation is often used in production. Grafted rose seedlings, because the rootstocks are mostly roses with developed roots, so the grafted rose seedlings will grow very fast in the first 1-2 years. It can be said that the growth rate of cuttings is 2-3 times faster.

However, because it is grafted, when the plant grows to the third year, it will begin to have the phenomenon of aging and decline in growth vitality. I have planted several grafted roses myself. I found that although the grafted rose grows fast in the early stage, it is not easy to produce bamboo shoots. Only in the first 1-2 years of planting, there are bamboo shoots, and the quantity is not much, and then rarely produce bamboo shoots. And many varieties of rose need to renew branches, which is why, after 3 years, the grafted seedlings will have the problem of aging, that is, many rose varieties need to renew branches, and the old branches have weak germination ability, which is not conducive to flowering.

Another is that the grafted rose seedlings, if the grafting technology does not pass, it is also very easy to grow the bamboo shoots of rootstocks in the process of culture, if the bamboo shoots grow out of rootstocks, and you can't tell them out and remove them in time, then this is a very depressing thing, because the bamboo shoots of rootstocks will seize and consume a lot of nutrients. So when you find that your grafted rose seedlings are sprouting from the soil, you should be careful, probably the buds of the rootstock.

2. Characteristics of cutting seedlings:

Compared with the grafted seedlings, the growth of the cutting rose seedlings is obviously not as fast as that of the grafted seedlings in the first 1-2 years, which is determined by the strong and weak sensation of the root system, which is easy to understand. However, after 2 years of growth, to the third year, the cutting rose will often burst into the rapid growth period, constantly sprouting bamboo shoots from the basic.

Due to the strong germination ability of shoot buds of cutting seedlings, the aged branches can be replaced at any time, so there is no problem of plant aging. Compared with the two, from the perspective of common interests, it is better to choose cuttings, after all, grafted seedlings still have hidden dangers of rootstock growth. And if you want a quick effect, then of course the first choice of grafted seedlings, fast growth, a short time can achieve a better flowering effect.

Therefore, it is best to choose what suits you according to your own needs.

Differences, advantages and disadvantages between rose cuttings and grafted seedlings

Flower friends in the selection of rose seedlings, do not know the difference between rose cutting seedlings and grafted seedlings, here we introduce the difference between rose cutting seedlings and grafted seedlings from different aspects to help flower friends choose reasonable rose seedlings.

1. Grafted rose seedlings

Some varieties of rose are difficult to root by cuttings, or their roots are underdeveloped. Using a strong rose as a rootstock, it can reproduce quickly and grow strong, with many flowers and large flowers.

Rose grafted seedlings can be divided into a variety of situations, such as the grafting seedlings of cutting rootstocks, the rootstocks also use cutting methods, at present, most of the domestic production is to cut out the rootstocks first, and then go to grafting to form new plants.

Another situation is the familiar imported seedlings, which belong to seed sowing seedlings and then grafted, which is characterized by inheriting the habits of the original rootstocks. At the same time, grafted seedlings also depend to a certain extent on the variety habits of rootstocks, such as growth cycle, resistance to diseases and insect pests and so on.

For the same variety, the way of grafting can not change the characteristics of the variety, for example, it grows fast, but in the early stage of grafting, the scion makes use of the strong absorption capacity of the rootstock to make it grow so fast that it can grow synchronously with the rootstock. When the root system and branches are balanced, the original characteristics of the variety will be restored. For example, slow-growing varieties will slow down to a certain extent. The use of rootstock grafting is often widely used, because batch precious varieties can be produced quickly. Those who are grafted grow quickly and age quickly.

According to the advantages and disadvantages of rose grafted seedlings, rose grafted seedlings generally grow more than 3 times faster than cutting seedlings, and they can develop into sturdy plants and produce standard flowers in the same year. The disadvantage is that the life span is short, the plants begin to senescence for more than 5 years, and the rootbuds often sprout.

Second, rose cuttings

Rose cuttings many flower friends know, perhaps some flower friends have tried, and successful, here we only briefly introduce some advantages and disadvantages, cutting seedlings belong to asexual reproduction, take its branches can take root to form an independent individual. Its character is consistent with the flower color, plant shape and habit of the female parent.

The advantages and disadvantages of rose cuttage seedlings, cutting seedlings do not have the problem of rootstock aging, rising vigorously in the later stage.

After reading the above content, I believe that many flower friends understand the difference between rose cuttings and grafting, so which one is more suitable for them? In fact, this depends on personal needs, the so-called radish and cabbage have their own preferences, some flower friends like immediate results, the first year to see flowers, some flower friends like the original. So in the end which kind of seedling to choose, I believe that flower friends can judge by themselves.

Fund project: technical specification for micro-grafting of tissue culture seedlings of Chinese rose

Rose is a common flower variety in people's daily life. with the development of flower culture technology, micro-grafting of rose tissue culture seedlings has become the research object of many agricultural experts. Today, the editor of Huinong Network is going to talk to you about a fund project: technical regulations for tissue culture seedlings micro-grafting of rose.

1 introduction

As one of the basic means of biotechnology, micro-grafting plays an important role in the progress of social science and technology. Micrografting is a combination of tissue culture and grafting, which is a grafting technique on rootstocks cultured in greenhouse or in test tubes. Now micro-grafting technology has been widely used in the research and production of fruit trees, flowers and trees.

Many excellent rose varieties have low seed setting rate, seeds are not easy to germinate or cuttings are difficult to take root. For these varieties, grafting is an efficient means of propagation.

2 range

This regulation stipulates the facilities related to rose micro-grafting, the selection and disinfection of substrate materials, the selection and disinfection of rootstocks and scions, the technical methods of micro-grafting, the management after grafting and so on. This procedure is applicable to the production of rose micro-grafting.

3 normative citation documents

The following documents are essential for the application of this document. The dated version applies to this document. For all undated reference documents, the latest version (including all amendment orders) applies to this document. Technical regulations for tissue culture and rapid propagation of flower seedlings in NY/T 2306mur2013.

4 rootstock and scion cultivation

4.1 selection of rootstocks

Rootstocks generally choose varieties with good growth and strong resistance to diseases and insect pests. The thornless rose can be selected as the rootstock of rose micro-grafting. Rootstocks can be propagated by cutting and tissue culture. The scions of rose micrografting are generally propagated by tissue culture.

4.2 cutting of rootstocks

The micrografting of rose can graft the scion cultured in the culture bottle to the rootstock in the greenhouse or the rootstock in the petri dish. Select the branches with full growth, elasticity and ungerminated flower buds in the current year. The upper part is cut into a flat mouth, and the lower part is cut into a Mal shape (about 45-60 °) at the 5~8mm under the node. The uppermost two leaflets are left in spring, summer and autumn, and the rest leaves are removed. All leaves should be removed when cutting in the greenhouse in winter. The matrix uses vermiculite or river sand, use cave pots or flowerpots, generally choose 50-hole hole pots. Generally, the cutting depth is 3~5cm, keep the plant spacing in 5~6cm with flowerpots or pots without holes, and then pour water thoroughly. Put the cut rose in a cool place, pay attention to moisturizing, if the temperature is not high, you can use plastic film to set up the arch shed. When the temperature is 20-28 ℃, it will take root in 30 days. After the rose has survived, there is no need to rush to transplant. The survival rate is higher when the roots are aged and turned brown.

4.3 tissue culture of rootstock and scion

4.3.1 explant treatment

The young stems of rose without diseases and insect pests were selected and washed under tap water for 3 hours. Except for leaves, cut the stem segment of 1.5cm with one bud per segment, first disinfect it with 70% alcohol for 15 seconds, then rinse it with sterile water for 3 times, then disinfect 10min with 2% effective concentration of NaClO and rinse with sterile water for 3 times. Put the sterilized stem bud upward.

The culture temperature was 24 ℃ ±2 ℃, the light intensity was 3000~4500lx and the light time was 12h/d.

4.3.2 subculture

The buds that initiated the culture and germination were cut off and inoculated on the subculture medium MS+6-BA2.0mg/L+ GA31.0mg/L. The culture temperature was 24 ℃ ±2 ℃, fluorescent light source, light intensity 3000~4500lx and light time 16h/d. When the tender stem of rose used as scion grows to 2~3cm, it can be grafted.

4.3.3 rooting culture

When the tender stem of the thornless rose as a rootstock grows to 2~3cm, the clustered seedlings are cut into plants and transferred to the rooting medium 1/2MS+IBA 1.0mg/L+NAA 0.5mg/L, and micro-grafting can be carried out after the seedlings take root.

5 grafting

5.1 preparation before grafting

Scissors, scalpels, tweezers and Parafilm film should be prepared before grafting. The rootstocks in the greenhouse need to be watered 1-2 days before grafting to ensure sufficient water. Before grafting, the rootstocks in the greenhouse can be sprayed with 500 times of streptomycin + 600 times of Chunleimycin to prevent bacterial damage.

5.2 grafting

The grafting method was split grafting. When the rootstock is tissue culture seedling: under aseptic condition, the rootstock which has grown root in rooting medium is selected, the top bud of tissue culture seedling is removed, the stem segment of 2cm is left, the bottom lateral bud is removed with tweezers, and a cut with depth of 0.2~0.5cm is made from the top of rootstock. The scion which is about 1cm in length and similar in thickness to that of the rootstock is selected, and its base is cut into a wedge (the length is similar to the cut depth of the rootstock). Insert the cut scion into the rootstock, make it fit with the rootstock as much as possible, and then fix it with Parafilm film. Put it into the tissue culture room to continue the culture. after the scion survived, the seedlings can be trained and transplanted.

When the rootstock is the cultivated seedling in the greenhouse, the strong scion tissue culture seedlings are selected and put into the seedling training room, and the tissue culture seedlings can be grafted after they adapt to the external environment. Grafting should be carried out in a shady environment. The rootstock should select the tender stem of the same year, remove the terminal bud, remove the lateral buds below the top, and make a longitudinal cut with a depth of 0.2~0.5cm from the top of the rootstock. The scion which is about 1cm in length and similar in thickness to that of the rootstock is selected, and its base is cut into a wedge (the length is similar to the cut depth of the rootstock). Insert the cut scion into the rootstock, make it fit with the rootstock as much as possible, and then fix it with Parafilm film.

Technical specification for micrografting of tissue culture seedlings of Chinese rose

6 Management after grafting

The grafted seedlings cultured in the culture dish can be put into the culture room to continue to cultivate. The grafted seedlings in the greenhouse need to maintain humidity within 4 days after grafting, and can be bagged or built in a shed for moisturization. at the same time, pay attention to shading the sun with a shading net, and gradually open the bagging or shed 5 days later, according to the standard that the seedlings do not wilt. Until it is managed in a normal environment.

The seedlings grafted in the petri dish can be trained after the scion has survived and fully grown. Put the petri dish into the seedling training room, loosen the lid first, and observe the seedlings. If there are signs of wilting, you need to tighten the lid again, taking the wilting ratio of the seedlings as the standard, and finally completely open the lid, and then you can transplant the seedlings into the pot.

The above is the information compiled by the editor of Huinong Network

Rose is a common flower variety in people's daily life. with the development of flower culture technology, micro-grafting of rose tissue culture seedlings has become the research object of many agricultural experts. Today, the editor of Huinong Network is going to talk to you about a fund project: technical regulations for tissue culture seedlings micro-grafting of rose.

1 introduction

As one of the basic means of biotechnology, micro-grafting plays an important role in the progress of social science and technology. Micrografting is a combination of tissue culture and grafting, which is a grafting technique on rootstocks cultured in greenhouse or in test tubes. Now micro-grafting technology has been widely used in the research and production of fruit trees, flowers and trees.

Many excellent rose varieties have low seed setting rate, seeds are not easy to germinate or cuttings are difficult to take root. For these varieties, grafting is an efficient means of propagation.

2 range

This regulation stipulates the facilities related to rose micro-grafting, the selection and disinfection of substrate materials, the selection and disinfection of rootstocks and scions, the technical methods of micro-grafting, the management after grafting and so on. This procedure is applicable to the production of rose micro-grafting.

3 normative citation documents

The following documents are essential for the application of this document. The dated version applies to this document. For all undated reference documents, the latest version (including all amendment orders) applies to this document. Technical regulations for tissue culture and rapid propagation of flower seedlings in NY/T 2306mur2013.

4 rootstock and scion cultivation

4.1 selection of rootstocks

Rootstocks generally choose varieties with good growth and strong resistance to diseases and insect pests. The thornless rose can be selected as the rootstock of rose micro-grafting. Rootstocks can be propagated by cutting and tissue culture. The scions of rose micrografting are generally propagated by tissue culture.

4.2 cutting of rootstocks

The micrografting of rose can graft the scion cultured in the culture bottle to the rootstock in the greenhouse or the rootstock in the petri dish. Select the branches with full growth, elasticity and ungerminated flower buds in the current year. The upper part is cut into a flat mouth, and the lower part is cut into a Mal shape (about 45-60 °) at the 5~8mm under the node. The uppermost two leaflets are left in spring, summer and autumn, and the rest leaves are removed. All leaves should be removed when cutting in the greenhouse in winter. The matrix uses vermiculite or river sand, use cave pots or flowerpots, generally choose 50-hole hole pots. Generally, the cutting depth is 3~5cm, keep the plant spacing in 5~6cm with flowerpots or pots without holes, and then pour water thoroughly. Put the cut rose in a cool place, pay attention to moisturizing, if the temperature is not high, you can use plastic film to set up the arch shed. When the temperature is 20-28 ℃, it will take root in 30 days. After the rose has survived, there is no need to rush to transplant. The survival rate is higher when the roots are aged and turned brown.

4.3 tissue culture of rootstock and scion

4.3.1 explant treatment

The young stems of rose without diseases and insect pests were selected and washed under tap water for 3 hours. Except for leaves, cut the stem segment of 1.5cm with one bud per segment, first disinfect it with 70% alcohol for 15 seconds, then rinse it with sterile water for 3 times, then disinfect 10min with 2% effective concentration of NaClO and rinse with sterile water for 3 times. Put the sterilized stem bud upward.

The culture temperature was 24 ℃ ±2 ℃, the light intensity was 3000~4500lx and the light time was 12h/d.

4.3.2 subculture

The buds that initiated the culture and germination were cut off and inoculated on the subculture medium MS+6-BA2.0mg/L+ GA31.0mg/L. The culture temperature was 24 ℃ ±2 ℃, fluorescent light source, light intensity 3000~4500lx and light time 16h/d. When the tender stem of rose used as scion grows to 2~3cm, it can be grafted.

4.3.3 rooting culture

When the tender stem of the thornless rose as a rootstock grows to 2~3cm, the clustered seedlings are cut into plants and transferred to the rooting medium 1/2MS+IBA 1.0mg/L+NAA 0.5mg/L, and micro-grafting can be carried out after the seedlings take root.

5 grafting

5.1 preparation before grafting

Scissors, scalpels, tweezers and Parafilm film should be prepared before grafting. The rootstocks in the greenhouse need to be watered 1-2 days before grafting to ensure sufficient water. Before grafting, the rootstocks in the greenhouse can be sprayed with 500 times of streptomycin + 600 times of Chunleimycin to prevent bacterial damage.

5.2 grafting

The grafting method was split grafting. When the rootstock is tissue culture seedling: under aseptic condition, the rootstock which has grown root in rooting medium is selected, the top bud of tissue culture seedling is removed, the stem segment of 2cm is left, the bottom lateral bud is removed with tweezers, and a cut with depth of 0.2~0.5cm is made from the top of rootstock. The scion which is about 1cm in length and similar in thickness to that of the rootstock is selected, and its base is cut into a wedge (the length is similar to the cut depth of the rootstock). Insert the cut scion into the rootstock, make it fit with the rootstock as much as possible, and then fix it with Parafilm film. Put it into the tissue culture room to continue the culture. after the scion survived, the seedlings can be trained and transplanted.

When the rootstock is the cultivated seedling in the greenhouse, the strong scion tissue culture seedlings are selected and put into the seedling training room, and the tissue culture seedlings can be grafted after they adapt to the external environment. Grafting should be carried out in a shady environment. The rootstock should select the tender stem of the same year, remove the terminal bud, remove the lateral buds below the top, and make a longitudinal cut with a depth of 0.2~0.5cm from the top of the rootstock. The scion which is about 1cm in length and similar in thickness to that of the rootstock is selected, and its base is cut into a wedge (the length is similar to the cut depth of the rootstock). Insert the cut scion into the rootstock, make it fit with the rootstock as much as possible, and then fix it with Parafilm film.

Technical specification for micrografting of tissue culture seedlings of Chinese rose

6 Management after grafting

The grafted seedlings cultured in the culture dish can be put into the culture room to continue to cultivate. The grafted seedlings in the greenhouse need to maintain humidity within 4 days after grafting, and can be bagged or built in a shed for moisturization. at the same time, pay attention to shading the sun with a shading net, and gradually open the bagging or shed 5 days later, according to the standard that the seedlings do not wilt. Until it is managed in a normal environment.

The seedlings grafted in the petri dish can be trained after the scion has survived and fully grown. Put the petri dish into the seedling training room, loosen the lid first, and observe the seedlings. If there are signs of wilting, you need to tighten the lid again, taking the wilting ratio of the seedlings as the standard, and finally completely open the lid, and then you can transplant the seedlings into the pot.

The above are the technical rules of micro-grafting of tissue culture seedlings arranged by Huinong net. Have you learned all these? With the continuous improvement and progress of culture technology, the technology of flower practitioners should also be constantly updated to learn, so as to make the flower culture industry better and better.