Cultivation techniques of hericium Erinaceus bag material

Hericium Erinaceus is a famous edible and medicinal fungus in China. The optimum temperature range of mycelium growth is 22 ℃ 24 ℃, and the best fruiting body growth stage is 18 Murray 20 min. When the bag material is cultivated, the air humidity is not strictly required in the mycelium growth stage, and the air relative humidity in the fruiting body growth stage is 85% Murray 90%. Annual production can be organized as long as the above temperature and humidity conditions are met. Prepare the culture material formula: 1. Cotton husk 78%, wheat bran 20%, sucrose 1%, gypsum 1%. 2. Corn (2344, 0.00,0.005%) core 78%, wheat bran 20%, sucrose 1%, gypsum 1%. 3. 40% of cotton husk, 30% of sawdust, 25% of wheat bran, 2% of corn meal, 1% of gypsum powder, 1% of sucrose, 1% of potassium dihydrogen phosphate. In production, one of the raw materials can be selected according to the supply of raw materials and production scale, which requires that the raw materials are fresh and mildew-free. After the raw materials are prepared, mix evenly in proportion, and then mix well with water, so that the water content of the culture material reaches 60% Murray 70%. The pH value is 4.5% Murray 6.5 (considering that the culture medium is gradually acidified in the future culture process, the pH value is 6.5), and then soak for about 30 minutes to bag. It should be noted that when using formula 2, the corncob should be crushed to the size of soybeans (4469,-1.00,-0.02%), soaked in water one day in advance, fully absorb water, remove the excess water before use, and then mix well with other ingredients. The prepared culture materials should not be accumulated for too long, especially in the high temperature season, otherwise they will deteriorate and cannot be used. Bagged sterilization inoculation culture material can be bagged after preparation, plastic (11310,-5.00,-0.04%) bag specification is 35 cm x 17 cm x 0.05 cm, polypropylene bag is selected for high pressure sterilization, polyethylene bag can be used for atmospheric pressure sterilization. Each bag contains about 450 grams of dry material, which is required to be packed loosely and tightly. After filling, put on a hard plastic neck ring and close the lid. At this time, the plastic bag, neck ring and lid should be checked for damage, and if there is damage, it needs to be replaced. There are two kinds of sterilization: atmospheric pressure sterilization and high pressure sterilization. Atmospheric pressure sterilization is sterilized under atmospheric pressure for 12 hours, and autoclave sterilization is sterilized under 1.5 kg / square centimeter pressure for 2 hours. After sterilization, take out the material bag, check again for damage and water bag, and then carry it into the inoculation room for vaccination. Inoculate under aseptic conditions in the inoculation box when the bag temperature is less than 30%. In order to shorten the inoculation time, two inoculations were used, that is, one at the mouth of the bag and one at the bottom of the bag. Close the lid quickly after receiving, and seal the hole tightly with duct tape or special sealing film. After all the bags are connected, label them and send them to the sterilization room for culture. Management of seedling stage 1. Temperature and humidity management. By means of sprinkling, shading and ventilation, the room temperature is controlled at 22 ℃ and the air relative humidity is controlled below 70%. 2. Light management. Because the growth stage of the mycelium of hericium Erinaceus does not need light, so the culture room should be shaded to keep dark. 3. Other management. About 4 days after moving into the sterilization room, the hyphae began to eat. At this time, check for contamination and pick out the contaminated bags. In addition, it is also necessary to check for the harm of rats and so on. Management of the occurrence period of fruiting body after 30 days of cultivation, the mycelium has been full of material bags or is about to be full of material bags. At this time, through ventilation and ventilation, the temperature of the culture room is reduced to 20 ℃, and the shading is removed to increase the scattered light. After about 7 days, when the buds begin to appear on the material surface, move the bacteria bag into the mushroom shed or adjust the temperature of the culture room to 18 Murray and 20 ℃, remove the lid of the bag and remove the collar to produce mushrooms. At this time, it is necessary to keep the air relative humidity at 85% Murray 90%, increase scattered light, and pay attention to proper ventilation, so that the fruit body can mature after 15 days. It can be harvested when the diameter of the fruit body of hericium Erinaceus reaches 5 Murray 10 cm or well-done. When harvesting, cut off the mushroom stalk with a knife and leave the stubble 1MUR 2cm to facilitate regeneration. After harvest, we will continue to strengthen the management of moisture, temperature and humidity, and new fruit bodies will grow in about 10 days. Generally, 3 Pleurotus ostreatus can be collected in each bacterial bag, and the bioconversion rate is 60% Murray 80%.