Indoor cultivation of hericium Erinaceus

The indoor cultivation methods of hericium Erinaceus include bottle cultivation and open cultivation, and the culture materials include sawdust and cottonseed skin. The optimum temperature for mycelium growth of hericium Erinaceus is 22-28 ℃, and the suitable temperature for fruiting body growth is 18-22 ℃. If the temperature is too low, the fruiting body grows slowly. The humidity requirement of mycelium is 60%, and that of fruiting body is 85.95%. First, the strain should be isolated first to cultivate hericium Erinaceus. There are two methods of strain isolation: spore isolation and tissue isolation. Spore isolation is to collect the spores of mature hericium Erinaceus under aseptic conditions and use them as raw materials for mycelium cultivation. Tissue separation is to take the fresh and tender fruiting body, put it into the inoculation box, cut the mushroom body under aseptic conditions, take a piece of tissue with good meat quality in the middle, about the size of rice grains, and put it on the inclined medium in the test tube. The culture medium was made by mixing peeled potato and cabbage with glucose in a ratio of 200 to 20, adding 1000 grams of water, adjusting the pH value to 5.5 to 6, then putting it into a glass tube, not filling it, sterilizing it under high pressure and forming a sloping surface. After inoculation, the test tube was cultured in a 25 ℃ incubator for 3 days, and the mycelium began to germinate at the end of the 20th to 25th day, when the mycelium covered the surface of the culture medium, the culture with good growth and no miscellaneous bacteria could be selected, and the cultivated species could be cultivated when the mycelium covered the slope of the test tube medium. 2. cultivation of cultivated species the culture materials of cultivated species can be sawdust (without aromatic oil or fungicide) or cottonseed skin, etc. the proportion of ingredients can be selected as follows: (1) sawdust 78%, wheat bran 20%, gypsum 1%, white sugar 1%; (2) cottonseed skin 98%, gypsum 1%, white sugar 1% (3) 80% of fresh distiller's grains, 10% of wheat bran, 9% of bran, 1% of gypsum powder (plus inorganic salt nutrient solution, the formula is: ammonium sulfate 3%, potassium dihydrogen phosphate 1%, magnesium sulfate 0.1%, adjusted to pH 4 with lactic acid. (4) mix the medium material with 60% water and squeeze the fingers with water stains instead of dripping. Then, fill the bottle to the shoulder of the bottle and sterilize it under 14.7 Newton pressure for 1 hour. After sterilization, use aseptic operation to pick up a piece of bevel bacteria, put it into the culture medium bottle, make the temperature reach 20: 25 ℃, the air relative humidity reaches 60% 65%, plug with cotton stopper. After about 30-40 days, the hyphae grew all over the culture material and formed a bacterial membrane. When the fruiting body primordium on the bacterial membrane is close to the cotton plug, remove the cotton plug. Third, the cultivation of fruit body open cultivation of hericium Erinaceus is to disinfect the sawdust of broad-leaved trees under high pressure, or boil it in a pot for 20 minutes, remove and drain the water, mix it directly into about 40% of the bacteria in the bottle after cooling, mix it into a plastic bag or wrap it in a film. The mouth of the bag or bandage was aerated with cotton plugs and cultured in a low temperature below 10 ℃. After growing into a bacterial mass, remove the film, and then continue to culture at natural temperature. Hericium Erinaceus can grow after a few months. The yield of this method is similar to that of bottle cultivation, but it can save bacteria and bottles and can be produced on a large scale. When the cotton stopper is opened, the room temperature of hericium Erinaceus is kept at 18-20 ℃. If the temperature is too high, close the doors and windows at noon and ventilate when the temperature is low in the morning and evening. If the temperature is low, it can be ventilated at noon. The indoor humidity is maintained at 85% 95%. When it is too low, it can splash water on the ground and keep it moist frequently. At the same time, it can be sprayed 3 times a day in space for 4 times, and a fruiting body can be formed after half a month. Compared with sawdust and cottonseed skin as culture material, cottonseed bark has the characteristics of faster bacteria, less miscellaneous bacteria and earlier fruiting body, which forms fruiting body about half a month earlier than sawdust. Fourth, the harvest fruiting body begins to atrophy and senescence stops growth and development, and the surface changes from white to brown, then it can be harvested and dried. If edible, it can be harvested before the spores are released to avoid the bitter taste of the spores. Under the same management conditions, three hericium Erinaceus can be harvested in each bottle, and the remaining mycelia can be used as pharmaceuticals.