Instructions for the use of foot-and-mouth disease vaccine, points for attention in the use of foot-and-mouth disease vaccine

A: cattle and sheep are immunized with bivalent inactivated vaccine against foot-and-mouth disease (FMD) type O-Asian type I.

Pigs: immunized with type O inactivated foot-and-mouth disease vaccine.

Foot-and-mouth disease vaccine is a kind of inactivated vaccine, as one of the most important weapons to prevent and cure the occurrence and epidemic of foot-and-mouth disease, the vaccine should be emulsion, allowing a small amount of oil phase precipitation or emulsion column stratification. If you can gently shake the emulsion to make the emulsion uniform, if you break the emulsion or exceed the prescribed delamination, you cannot use it. Let's take a look at how to use the foot-and-mouth disease vaccine.

Inactivated bovine type O foot-and-mouth disease vaccine

The inactivated bovine foot-and-mouth disease vaccine type O was selected as the virulent bovine virus OA/58 with broad antigenic spectrum, good antigenicity and immunogenicity, and inoculated into the monolayer culture of BHK-21 cell line. The virus antigen was inactivated by diethyleneimine (BEI) and added mineral oil adjuvant. It was a slightly pink or milky sticky liquid. It is used for vaccination and emergency vaccination of yellow cattle, buffalo, dairy cows and yaks of all ages. The immunization duration is 6 months. Adult cattle were injected with 3 ml intramuscularly, and calves under 1 year old were injected with 2 ml intramuscular injection. This product should be protected from freezing and stored under the condition of 4-8 ℃ with a validity period of 10 months.

Swine type O foot-and-mouth disease inactivated vaccine

The inactivated vaccine of swine type O foot-and-mouth disease was prepared by inoculating BHK-21 or IBRS-2 cell monolayer with virulent pig virus, harvesting cytovenom, inactivating with diethyleneimine (BEI) and emulsifying with oil adjuvant. It is a milky white or reddish viscous emulsion. After storage, a small amount of oil is allowed on the liquid surface and a small amount of water at the bottom of the bottle (no more than 1 to 10 respectively). Shake it into a uniform emulsion. It is used to prevent type O foot-and-mouth disease in pigs, and the immune duration is 6 months. Shake the vaccine well before injection. Guohao foot-and-mouth Ling is injected into the muscle behind the pig's ear root. The body weight is 10 to 25 kilograms and 2 milliliters are injected. 3 milliliters are injected over 25 kilograms. Note that the vaccine should be refrigerated and shipped below 10 ℃. This product is stored in 2-10 ℃ cold storage and is valid for 1 year.

Foot-and-mouth disease type O attenuated live vaccine

The live attenuated vaccine of foot-and-mouth disease type O was inoculated into suckling rabbits with type O attenuated strain of foot-and-mouth disease, the poisonous tissue was harvested and ground, and the virus extract was made by adding the same amount of glycerol, which was a dark red liquid. After static placement, there was partial precipitation at the bottom of the bottle. Shake and give a uniform suspension. It is used to prevent type O foot-and-mouth disease in yellow cattle and yaks over 1 year old and sheep and goats over 4 months old. The immune duration was 6-8 months after intramuscular injection of 2 ml and subcutaneous injection of 1 ml of sheep. This product can not be used in pigs, cows and buffaloes. The period of validity is 1 year under-12 ℃, 5 months at 2-6 ℃ and 7 days at 20-22 ℃.

Matters needing attention in the use of foot-and-mouth disease vaccine

1. Syringes and needles should be clean and sterilized with hot and humid methods under high pressure or boiled with clean water for at least 15 minutes. Chemical disinfection should not be used, needles should be changed head by head during vaccination, and one syringe should not be used for mixed use of two kinds of vaccines.

2. The animal breed, health status, medical history and immune history should be known before vaccination. All sick, thin and parturient female animals (10-15 days) should not be vaccinated, and should be replenished according to the regulations when the sick animals recover and the mothers give birth. Vaccinated animals should be kept at rest, under quiet conditions and kept clean. Livestock in non-epidemic areas can not be transported until 28 days after injection.

3. During use, the vaccine should be kept at a low temperature and avoid direct sunlight. After shearing, the injection site should be cleaned and disinfected with 70% alcohol cotton or iodine tincture, and then dry and disinfect the site with squeezed alcohol cotton. The vaccine must be injected into the muscle. Do not inject fat or subcutaneous.

4. Strengthen the feeding and management of animals after vaccination, and it is a normal reaction to stop eating or reduce food for 1 or 2 days. A small number of livestock may have vaccine allergic reactions due to breeds and individual conditions. observation should be strengthened and emergency treatment with epinephrine or other methods should be used in time to reduce losses.